The DOI matches its Crossref record; 56 of the 58 references it cites were found in a registry or an index.
Anabolic Effects of 1,25-Dihydroxyvitamin D3 on Osteoblasts Are Enhanced by Vascular Endothelial Growth Factor Produced by Osteoblasts and by Growth Factors Produced by Endothelial Cells*
Abstract
Human osteoblast-like cells (HOB) produce vascular endothelial growth factor (VEGF), the steady state level of which is stimulated by 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]. As osteoblasts and endothelial cells are proximally located in skeletal tissue, we investigated the anabolic effects of 1,25-(OH)2D3 and VEGF on HOB cocultured with endothelial cells. When HOB with high alkaline phosphatase (Al-P) activity and human umbilical vein endothelial cells (HUVEC) with little activity were cultured together, Al-P activity increased, accompanied by an increase in cell number. When HOB and HUVEC were cultured separately, 1,25-(OH)2D3 did not directly stimulate [3H]thymidine incorporation into HUVEC, but stimulated it in the presence of HOB. VEGF did not directly stimulate the Al-P activity of HOB but stimulated it in the presence of HUVEC. The conditioned medium of HOB stimulated the proliferation of HUVEC, and this was partially blocked by anti-VEGF antibody. Conversely, the conditioned medium of HUVEC increased Al-P activity and [3H]thymidine incorporation into HOB, and this was partially blocked by antiinsulin-like growth factor I antibody and BQ-123, a specific antagonist of the endothelin-1 (ET-1) receptor. 1,25-(OH)2D3 stimulated the release of VEGF and ET-1 from HOB and HUVEC, respectively. Furthermore, the 1,25-(OH)2D3-induced release of VEGF was enhanced in HOB cocultured with HUVEC. A quantitative reverse transcription-PCR study revealed that genes for VEGF receptors (Flt-1 and KDR) were expressed in HUVEC, but not in HOB, and that 1,25-(OH)2D3 increased the levels of expression of VEGF receptor genes in endothelial cells only when cocultured with HOB. In summary, we demonstrated that 1,25-(OH)2D3 exerts an anabolic effect on osteoblasts by enhancing their production of VEGF, which stimulates its receptors on endothelial cells, followed by increased production of osteotropic growth factors, such as insulin-like growth factor I and ET-1. These in vitro findings suggest that the VEGF/VEGF receptor system may be involved in both bone formation and bone remodeling in vivo.
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“Additionally, VEGF prompts MSC migration and triggers osteoblast differentiation through osteotropic growth factors secreted by activated endothelial cells [39,84].”
From Mesenchymal Stem/Stromal Cells: Immunomodulatory and Bone Regeneration Potential after Tumor Excision in Osteosarcoma Patients (2023) · cites this paper as [84]
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Cites
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,515
(2019) · Elsevier eBooks · doi:10.1016/c2015-1-01622-2 · Cited by 1,690
Molecular and Biological Properties of the Vascular Endothelial Growth Factor Family of Proteins
Ferrara, N., Houck, K. A., Jakeman, L. B., & LEUNG, D. W. (1992) · Endocrine Reviews · doi:10.1210/edrv-13-1-18 · Cited by 1,655
Endothelins: molecular biology, biochemistry, pharmacology, physiology, and pathophysiology.
Rubanyi, G. M., & Polokoff, M. A. (1994) · Pharmacological Reviews · doi:10.1016/s0031-6997(25)06809-7 · Cited by 1,645
Compston, J. E. (1997) · Clinical Endocrinology · doi:10.1046/j.1365-2265.1997.2431019.x · Cited by 1,383
The Role of the Vitamin D Endocrine System in Health and Disease
Reichel, H., Koeffler, H. P., & Norman, A. W. (1989) · New England Journal of Medicine · doi:10.1056/nejm198904133201506 · Cited by 1,037
Shibuya, M., Yamaguchi, S., Yamane, A., Ikeda, T., Tojo, A., Matsushime, H., & Sato, M. (1990) · PubMed · Cited by 960
Quinn, T. P., Peters, K. G., de Vries, C. J. M., Ferrara, N., & Williams, L. T. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.16.7533 · Cited by 754
Toward a molecular understanding of skeletal development
Erlebacher, A., Filvaroff, E. H., Gitelman, S. E., & Derynck, R. (1995) · Cell · doi:10.1016/0092-8674(95)90487-5 · Cited by 690
Vascular endothelial growth factor and its receptors
Klagsbrun, M., & D'Amore, P. A. (1996) · Cytokine & Growth Factor Reviews · doi:10.1016/s1359-6101(96)00027-5 · Cited by 683
Research works printed but not linked: 1
- Canalis, 1994, The Parathyroids, 65
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Carano, R. A. D., & Filvaroff, E. H. (2003) · Drug Discovery Today · doi:10.1016/s1359-6446(03)02866-6 · Cited by 759
Deckers, M. M. L., van Bezooijen, R. L., van der Horst, G., Hoogendam, J., van der Bent, C., Papapoulos, S. E., & Löwik, C. W. G. M. (2002) · Endocrinology · doi:10.1210/endo.143.4.8719 · Cited by 651
Biofabrication of bone tissue: approaches, challenges and translation for bone regeneration
Tang, D., Tare, R. S., Yang, L.-Y., Williams, D. F., Ou, K.-L., & Oreffo, R. O. C. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.01.024 · Cited by 613
Effect of local sequential VEGF and BMP-2 delivery on ectopic and orthotopic bone regeneration
Kempen, D. H. R., Lu, L., Heijink, A., Hefferan, T. E., Creemers, L. B., Maran, A., Yaszemski, M. J., & Dhert, W. J. A. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.01.031 · Cited by 614
Deckers, M. M. L., Karperien, M., van der Bent, C., Yamashita, T., Papapoulos, S. E., & Löwik, C. W. G. M. (2000) · Endocrinology · doi:10.1210/endo.141.5.7458 · Cited by 550
Metadata
- Authors
- Wang, D. S., Miura, M., Demura, H., & Sato, K.
- Affiliations
- Tokyo Women's Medical University (JP)
- Mitsubishi Kagaku BCL (M.M.), Itabashi-ku, Tokyo, Japan
- Corresponding author
- Sato, Kanji
- ORCID
- Miura, Masakazu
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1997-07-01
- Volume
- 138
- Issue
- 7
- Pages
- 2953-2962
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2019113771
- Cited by
- 254
- References
- 58
- Citations by year
- 2025: 3 · 2024: 1 · 2023: 9 · 2022: 4 · 2021: 5 · 2020: 2 · 2019: 12 · 2018: 9 · 2017: 4 · 2016: 9 · 2015: 8 · 2014: 13 · 2013: 16 · 2012: 10
- FWCI
- 2.02
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Vitamin D Research Studies
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Keywords
- 1,25-dihydroxyvitamin D3, vascular endothelial growth factor, osteoblasts, endothelial cells, human umbilical vein endothelial cells, endothelin-1, insulin-like growth factor I, VEGF receptors, Flt-1, KDR, alkaline phosphatase activity, bone formation, bone remodeling, endothelial cell proliferation, conditioned medium, BQ-123, anti-VEGF antibody, gene expression
- MeSH terms
- Alkaline Phosphatase, Calcitriol, Endothelium, Vascular, Humans, Lymphokines, Models, Biological, Osteoblasts, Receptors, Mitogen, Endothelial Growth Factors, Receptors, Growth Factor, Coculture Techniques, Endothelin-1, Endothelin-2, Endothelin-3, Receptor Protein-Tyrosine Kinases, Receptors, Vascular Endothelial Growth Factor, Vascular Endothelial Growth Factors, Vascular Endothelial Growth Factor A
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