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Cited by 113Bone Metabolism and DiseasesBone health and osteoporosis researchOsteomyelitis and Bone Disorders Research

The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.

Dexamethasone Promotes Expression of Membrane-Bound Macrophage Colony-Stimulating Factor in Murine Osteoblast-Like Cells 1

Rubin, J., Biskobing, D. M., Jadhav, L., Fan, D., Nanes, M. S., Perkins, S., Fan, X.·1998·Endocrinology
DOI 10.1210/endo.139.3.5778
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Abstract

The mechanisms by which glucocorticosteroids promote osteoclastogenesis in vitro are uncertain. As macrophage colony-stimulating factor (MCSF) is critical for osteoclastogenesis, we hypothesized that glucocorticosteroids might regulate membrane-bound MCSF (mMCSF) and soluble MCSF (sMCSF) production by stromal cells or osteoblasts. ST2 cells or murine calvarial osteoblasts (MOBs) were treated with dexamethasone (Dex; 100 nM) and/or 1,25-dihydroxyvitamin D [1,25(OH)2D; 10 nM] for 3 days. Control values for mMCSF and sMCSF as units per 100,000 cells were 9 +/- 1.4 and 511 +/- 56 in ST2 cells and 5.9 +/- 0.8 and 379 +/- 47 in MOB cells, respectively. Dex increased mMCSF to 156 +/- 16% and 143 +/- 26% compared with the control value in ST2 and MOB cells, respectively, whereas 1,25-(OH)2D caused increases of 195 +/- 16% and 164 +/- 21%. In the presence of both Dex and 1,25-(OH)2D, mMCSF increased to 209 +/- 24% and 216 +/- 26% in the two cell types, respectively. 1,25-(OH)2D caused modest increases in sMCSF, as expected, in both cell types (153 +/- 6% and 122 +/- 4%). Dex inhibited 1,25-(OH)2D-stimulated sMCSF (115 +/- 7% of control) in ST2 cells. Analysis of mMCSF transcript levels by semiquantitative RT-PCR revealed Dex-stimulated increases of 170 +/- 11% in ST2 cells and 126 +/- 16% in MOB cells compared with the control level. The increased expression of the transcript for sMCSF in the presence of Dex and 1,25-(OH)2D, measured by both RT-PCR and Northern analysis (219 +/- 53% and 242%, respectively), despite inhibition of sMCSF protein, indicated that the inhibitory effect of Dex in ST2 cells was posttranscriptional. Half-life studies showed that Dex prolonged MCSF messenger RNA from 2.8 to 7.5 h. These results suggest that Dex influences osteoclastogenesis by increasing the expression of mMCSF by accessory cells in culture.

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Crossref · 10.1210/endo.139.3.5778

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Metadata

Authors
Rubin, J., Biskobing, D. M., Jadhav, L., Fan, D., Nanes, M. S., Perkins, S., & Fan, X.
Affiliations
  • Emory University (US)
  • University of Utah (US)
Corresponding author
Rubin, J.
ORCID
Venue
Endocrinology
Publisher
The Endocrine Society
Published
1998-03
Volume
139
Issue
3
Pages
1006-1012
ISSN
  • 0013-7227 (ISSN-L)
  • 1945-7170
DOI
10.1210/endo.139.3.5778
OpenAlex
W2080570046
Cited by
113
References
37
Citations by year
2026: 1 · 2025: 3 · 2023: 2 · 2022: 3 · 2021: 5 · 2020: 3 · 2019: 4 · 2018: 4 · 2017: 4 · 2016: 2 · 2015: 5 · 2014: 7 · 2013: 4 · 2012: 5
FWCI
1.74
Type
journal article
Language
en
Open access
closed
Locations
  • Endocrinology (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR42360)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
  • Health Sciences › Medicine › Rheumatology › Osteomyelitis and Bone Disorders Research
Keywords
dexamethasone, macrophage colony-stimulating factor, osteoclastogenesis, glucocorticoids, 1,25-dihydroxyvitamin D, osteoblast-like cells, ST2 cells, calvarial osteoblasts, osteoblasts, gene expression regulation, bone resorption
MeSH terms
Animals, Cells, Cultured, Dexamethasone, Dose-Response Relationship, Drug, Enzyme-Linked Immunosorbent Assay, Mice, Inbred C57BL, Osteoblasts, RNA, Messenger, Macrophage Colony-Stimulating Factor, Mice

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