The DOI matches its Crossref record; 59 of the 61 references it cites were found in a registry or an index.
Expression and activity of 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase in the rat Purkinje neuron during neonatal life
Abstract
Recently, we demonstrated that cytochrome P450 side-chain cleavage enzyme (P450scc) occurs in the rat cerebellar Purkinje cell after differentiation and remains during neonatal development and into adulthood. 3Beta-hydroxysteroid dehydrogenase/delta5-delta4-isomerase (3betaHSD) is also an essential enzyme for progesterone biosynthesis not only in peripheral steroidogenic glands but also in the nervous system. In the present study, therefore, the expression of 3betaHSD in the rat cerebellum was investigated during neonatal development and in the adult. RT-PCR analysis showed that the expression of 3betaHSD messenger RNA (mRNA) in the cerebellum was higher at 7-14 days of age than at other times. Biochemical studies together with HPLC analysis revealed that cerebellar slices at 10 days of age converted pregnenolone to progesterone, suggesting enzymatic activity of 3betaHSD. This conversion was significantly reduced by trilostane, a specific inhibitor of 3betaHSD. A specific RIA indicated that progesterone concentrations in the cerebellum were higher at 3 and 10 days of age than at 60 days of age. The progesterone level in the cerebellum was significantly higher than that in plasma at 10 days of age. In contrast, the concentrations in both cerebellum and plasma at 3 and 60 days of age were similar. In the present study, the site of 3betaHSD mRNA expression in the cerebellum was further examined in neonatal and adult rats using in situ hybridization. The cerebellar expression of 3betaHSD mRNA was obscure at 3 days of age, whereas intense expression occurred in Purkinje cells and external granule cells throughout the cerebellum at 10 days of age. 3BetaHSD mRNA was also expressed in Purkinje cells and granule cells at 60 days of age, but a restricted expression was observed along the cerebellar meninges. These results suggest that the steroidogenic enzyme 3betaHSD as well as P450scc are expressed at least in the cerebellar Purkinje cell. The expression of 3betaHSD, however, may increase for a limited period around 10 days of age, unlike P450scc.
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Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,515
Steroid hormone metabolites are barbiturate-like modulators of the GABA receptor
Majewska, M. D., Harrison, N. L., Schwartz, R. D., Barker, J. L., & Paul, S. M. (1986) · Science · doi:10.1126/science.2422758 · Cited by 2,225
Paul, S. M., & Purdy, R. H. (1992) · The FASEB Journal · doi:10.1096/fasebj.6.6.1347506 · Cited by 1,413
Molecular biology of steroid hormone synthesis
MILLER, W. L. (1988) · Endocrine Reviews · doi:10.1210/edrv-9-3-295 · Cited by 1,397
Majewska, M. D. (1992) · Progress in Neurobiology · doi:10.1016/0301-0082(92)90025-a · Cited by 1,089
The nucleotide sequence of the rat cytoplasmic β–actin gene
Nudel, U., Zakut, R., Shani, M., Neuman, S., Levy, Z., & Yaffe, D. (1983) · Nucleic Acids Research · doi:10.1093/nar/11.6.1759 · Cited by 1,025
Altman, J. (1972) · Journal of Comparative Neurology · doi:10.1002/cne.901450402 · Cited by 996
Altman, J. (1972) · The Journal of Comparative Neurology · doi:10.1002/cne.901450305 · Cited by 912
Characterization and measurement of dehydroepiandrosterone sulfate in rat brain
Corpéchot, C., Robel, P., Axelson, M., Sjövall, J., & Baulieu, E. E. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.8.4704 · Cited by 656
(1997) · PubMed · Cited by 643
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- Tsutsui, 1997, P450scc activity in the brain, 427
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The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders
Miller, W. L., & Auchus, R. J. (2011) · Endocrine Reviews · doi:10.1210/er.2010-0013 · Cited by 2,458
Multiple Actions of Steroid Hormones—A Focus on Rapid, Nongenomic Effects
Falkenstein, E., Tillmann, H.-C., Christ, M., Feuring, M., & Wehling, M. (2000) · Pharmacological Reviews · doi:10.1016/s0031-6997(24)01468-6 · Cited by 1,002
Neurosteroids: biosynthesis and function of these novel neuromodulators
Compagnone, N. A., & Mellon, S. H. (2000) · Frontiers in Neuroendocrinology · doi:10.1006/frne.1999.0188 · Cited by 820
do Rego, J. L., Seong, J. Y., Burel, D., Leprince, J., Luu-The, V., Tsutsui, K., Tonon, M., Pelletier, G., & Vaudry, H. (2009) · Frontiers in Neuroendocrinology · doi:10.1016/j.yfrne.2009.05.006 · Cited by 365
Self-citationSchumacher, M., Mattern, C., Ghoumari, A. M., Oudinet, J., Lière, P., Labombarda, F., Sitruk-Ware, R. L., Nicola, A. F. D., & Guennoun, R. (2013) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2013.09.004 · Cited by 368
Metadata
- Authors
- Ukena, K., Kohchi, C., & Tsutsui, K.
- Affiliations
- Hiroshima University (JP)
- Corresponding author
- Tsutsui, Kazuyoshi
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1999-02-01
- Volume
- 140
- Issue
- 2
- Pages
- 805-813
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2046524470
- Semantic Scholar
- 3e1088688415429d5478a3e54657be8a7c93ab31
- Cited by
- 141
- References
- 61
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 5 · 2020: 3 · 2019: 6 · 2018: 2 · 2017: 3 · 2016: 8 · 2015: 1 · 2014: 6 · 2013: 3 · 2012: 4
- FWCI
- 2.88
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Central Lancashire
- Japan Society for the Promotion of Science
- Topics
- Physical Sciences › Chemistry › Organic Chemistry › Steroid Chemistry and Biochemistry
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Sexual Differentiation and Disorders
- Keywords
- 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase, Purkinje cells, neonatal development, progesterone biosynthesis, pregnenolone, trilostane, mRNA expression
- MeSH terms
- Aging, Animals, Animals, Newborn, Cerebellum, Male, Progesterone, Purkinje Cells, Radioimmunoassay, Rats, Inbred F344, RNA, Messenger, 17-Hydroxysteroid Dehydrogenases, In Situ Hybridization, Rats
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