3,5-Diiodo-l-Thyronine Regulates Glucose-6-Phosphate Dehydrogenase Activity in the Rat*
Abstract
Thyroid hormones influence the activity of lipogenic enzymes such as malic enzyme (ME) and glucose-6-phosphate dehydrogenase (G6PD). The effect of T3 on ME is exerted at the transcriptional level, but it is unclear if its effect on G6PD is also nuclear mediated. Furthermore, other iodothyronines that have been shown to possess biological activity (such as diiodothyronines) could contribute to this enzyme's regulation. In this study the effects of 3,5-diiodothyronine (T2) on the aforementioned enzymes were examined and compared with those of T3. Rats made hypothyroid by propylthiouracil and iopanoic acid treatment were used throughout. Enzyme activities were determined spectrophotometrically, and G6PD messenger RNA (mRNA) expression was analyzed by Northern blotting using a human G6PD complementary DNA probe. Injections of T2 to hypothyroid animals significantly enhanced the activity of both enzymes. The effect of T2 on ME was nuclear mediated and mimicked the effect of T3. The effects of T2 and T3 on G6PD differed. Injection of T3 into hypothyroid rats induced an increase in both enzyme activity and G6PD mRNA expression, indicating a nuclear-mediated effect. The effect of T2 on G6PD activity, on the other hand, was not nuclear mediated. The injection of T2 into hypothyroid animals did not change G6PD mRNA expression, and the strong increase in the enzyme's activity (from +70% to +300%) was unaffected by simultaneous injection of protein synthesis inhibitors. As the lowest dos…
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3,5-T2 affects the activity of enzymes involved in energy metabolism.
“and affects the activity of enzymes involved in energy metabolism, including α-glycerophosphate dehydrogenase (Lombardi et al. 2000), cytochrome oxidase (Lanni et al. 1992, 1993, Goglia et al. 1994, Arnold et al. 1998), malate dehydrogenase (Ball et al. 1997, Lombardi et al. 2000), glucose-6-phosphate dehydrogenase (Lombardi et al. 2000), and F0F1-ATP synthase (Mangiullo et al. 2010).”
From Administration of 3,5-diiodothyronine (3,5-T2) causes central hypothyroidism and stimulates thyroid-sensitive tissues (2014) · cites this paper as Lombardi et al. 2000
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Metabolic Effects of Thyroid Hormone Derivatives
Moreno, M., de Lange, P., Lombardi, A., Silvestri, E., Lanni, A., & Goglia, F. (2008) · Thyroid · doi:10.1089/thy.2007.0248 · Cited by 226 · see citing works
Self-citationPadron, A. S., Neto, R. A. L., Pantaleão, T. U., de Souza dos Santos, M. C., Araujo, R. L., de Andrade, B. M., da Silva Leandro, M., de Castro, J. P. S. W., Ferreira, A. C. F., & de Carvalho, D. P. (2014) · Journal of Endocrinology · doi:10.1530/joe-13-0502 · Cited by 69 · see citing works
- Authors
- Lombardi, A., Beneduce, L., Moreno, M., Diano, S., Colantuoni, V., Ursini, M. V., Lanni, A., & Goglia, F.
- Affiliations
- University of Naples Federico II (IT)
- University of Sannio (IT)
- Yale University (US)
- Institute of Genetics and Biophysics (IT)
- University of Campania "Luigi Vanvitelli" (IT)
- Corresponding author
- Goglia, F.
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 2000-05-01
- Volume
- 141
- Issue
- 5
- Pages
- 1729-1734
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2030802768
- Cited by
- 49
- References
- 0
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2019: 3 · 2018: 1 · 2016: 3 · 2015: 3 · 2014: 6 · 2013: 3 · 2012: 3
- FWCI
- 1.67
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Archivio Istituzionale della Ricerca - Università degli Studi della Campania "Luigi Vanvitelli" (submittedVersion)
- Funders
- Regione Campania
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Disorders and Treatments
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Keywords
- 3,5-diiodothyronine, triiodothyronine, glucose-6-phosphate dehydrogenase, malic enzyme, hypothyroidism, lipogenesis, iodothyronines
- MeSH terms
- Animals, Diiodothyronines, Glucosephosphate Dehydrogenase, Glycerolphosphate Dehydrogenase, Humans, Malate Dehydrogenase, Male, Triiodothyronine, Rats, Wistar, Rats
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