Corticosteroid-Binding Globulin Synthesis Regulation by Cytokines and Glucocorticoids in Human Hepatoblastoma-Derived (HepG2) Cells1
Abstract
Plasma corticosteroid-binding globulin (CBG) concentrations decrease dramatically in patients with septic shock or burn injury. This decrease suggests that mediators of the acute phase response, such as cytokines and glucocorticoid hormones, might influence clearance as well as liver synthesis of CBG in humans. The present study investigated the effects of interleukin-6 (IL-6), IL-1 beta, and dexamethasone on CBG synthesis by a clone of human hepatoblastoma-derived (HepG2) cell line. In culture medium from HepG2 cells, the immunoconcentration of CBG and the levels of CBG messenger ribonucleic acid (mRNA) were dose dependently decreased in the presence of IL-6 concentrations ranging from 0.1-10 ng/mL. The percent decrease in CBG immunoconcentration was quantitatively similar to the percent decrease in CBG mRNA levels (29 +/- 6% and 39 +/- 15%, respectively, of control values). In contrast, and as expected, IL-6 dose dependently increased the mRNA levels (164 +/- 22% of control values) of alpha 1-antitrypsin, a positive acute phase protein, but did not affect the immunoconcentration of sex hormone-binding globulin, another liver protein. Dexamethasone alone did not significantly affect CBG secretion or mRNA levels, but did dose-dependently increase tyrosine amino-transferase mRNA levels, which increased to 252 +/- 16% of the control values. However, in combination with IL-6, dexamethasone had a significant additive effect on IL-6 inhibition of CBG secretion and mRNAs in HepG2 c…
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Cytokines, including IL-6, have been shown to inhibit CBG production.
“Cytokines, including IL-6, have been shown to inhibit CBG production”
From Cytokines and Glucocorticoid Receptor Signaling (2009) · cites this paper as [82]
Cytokine-induced decreases in CBG are believed to play a role in the decreased levels of CBG seen in septic shock and burn patients.
“cytokine-induced decreases in CBG are believed to play a role in the decreased levels of CBG seen in septic shock and burn patients.81,82”
From Cytokines and Glucocorticoid Receptor Signaling (2009) · cites this paper as [82]
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- Authors
- Emptoz-Bonneton, A., Crave, J. C., Lejeune, H., Brébant, C., & Pugeat, M.
- Affiliations
- Hospices Civils de Lyon (FR)
- Venue
- The Journal of Clinical Endocrinology & Metabolism
- Publisher
- The Endocrine Society
- Published
- 1997-11
- Volume
- 82
- Issue
- 11
- Pages
- 3758-3762
- ISSN
- 0021-972X (ISSN-L)
- 1945-7197
- OpenAlex
- W1984952449
- Cited by
- 68
- References
- 0
- Citations by year
- 2025: 2 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 1 · 2018: 3 · 2017: 3 · 2016: 5 · 2014: 4 · 2013: 2 · 2012: 2
- FWCI
- 1.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Clinical Endocrinology & Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Funders
- McGill University
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
- Keywords
- corticosteroid-binding globulin, interleukin-6, IL-1β, dexamethasone, glucocorticoids, acute phase response, septic shock, burns, HepG2 cells, alpha-1-antitrypsin, tyrosine aminotransferase, hepatic protein synthesis
- MeSH terms
- Dexamethasone, Drug Interactions, Gene Expression Regulation, Glucocorticoids, Humans, Interleukin-1, Liver Neoplasms, RNA, Messenger, Transcortin, Tumor Cells, Cultured, Interleukin-6, Cytokines, Culture Media, Conditioned, Hepatoblastoma
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