CoChat
Sign Up
Cited by 160Prostate Cancer Treatment and ResearchProtein Kinase Regulation and GTPase SignalingEstrogen and related hormone effects

The DOI matches its Crossref record; 74 of the 74 references it cites were found in a registry or an index.

A Rapid, Nongenomic, Signaling Pathway Regulates the Actin Reorganization Induced by Activation of Membrane Testosterone Receptors

Papakonstanti, Evangelia A., Kampa, Marilena, Castanas, Elias, Stournaras, Christos·2003·Molecular Endocrinology
DOI 10.1210/me.2002-0253
Open this paper in CoChat — free account

Abstract

The human prostate cancer cell line LNCaP bears functional membrane testosterone receptors, which modify the actin cytoskeleton and increase the secretion of prostate-specific antigen (PSA) within minutes. Membrane steroid receptors are, indeed, a newly identified element of steroid action that is different from the classical intracellular sites. In the present work, using a nonpermeable analog of testosterone (testosterone-BSA), we investigated the signaling pathway that is triggered by the membrane testosterone receptors' activation and leads to actin cytoskeleton reorganization. We report that exposure of cells to testosterone-BSA resulted in phosphorylation of focal adhesion kinase (FAK), the association of FAK with the phosphatidylinositol-3 (PI-3) kinase, and the subsequent activation of the latter as well as the activation of the small guanosine triphosphatases Cdc42/Rac1. Pretreatment of cells with the specific PI-3 kinase inhibitor wortmannin abolished both the activation of the small guanosine triphosphatases and the alterations of actin cytoskeleton, whereas it did not affect the phosphorylation of FAK. These findings indicate that PI-3 kinase is activated downstream of FAK and upstream of Cdc42/Rac1, which subsequently regulate the actin organization. Moreover, wortmannin diminished the secretion of PSA, implying that the signaling events described above are responsible for the testosterone-BSA-induced PSA secretion. Our results are discussed under the prism of a possible implication of these membrane receptors in prostate cancer chemotherapy.

Overview

Integrity signals

Registry

Crossref · 10.1210/me.2002-0253

Cites

74 of 74 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Cited by

Sorted by citations

See all 160

Related

See all

Metadata

Authors
Papakonstanti, E. A., Kampa, M., Castanas, E., & Stournaras, C.
Affiliations
University of Crete (GR)
Corresponding author
Stournaras, Christos
ORCID
Venue
Molecular Endocrinology
Publisher
The Endocrine Society
Published
2003-05
Volume
17
Issue
5
Pages
870-881
ISSN
  • 0888-8809 (ISSN-L)
  • 1944-9917
DOI
10.1210/me.2002-0253
OpenAlex
W2059939225
Cited by
160
References
74
Citations by year
2026: 3 · 2025: 3 · 2024: 4 · 2023: 7 · 2022: 3 · 2021: 4 · 2020: 3 · 2019: 6 · 2018: 4 · 2017: 11 · 2016: 4 · 2015: 2 · 2014: 10 · 2013: 7 · 2012: 6
FWCI
8.10
Type
journal article
Language
en
Open access
closed
Locations
  • Molecular Endocrinology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Olympus
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Prostate Cancer Treatment and Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
Keywords
non-genomic steroid action, actin cytoskeleton remodeling, focal adhesion kinase, phosphatidylinositol 3-kinase, Cdc42, Rac1, small GTPases, prostate-specific antigen secretion, wortmannin, prostate cancer, LNCaP cells
MeSH terms
Wortmannin, Phosphoinositide-3 Kinase Inhibitors, Actins, Androstadienes, Cell Membrane, Cytoskeleton, Enzyme Inhibitors, Humans, Male, Neuropeptides, Phosphorylation, Prostatic Neoplasms, Protein-Tyrosine Kinases, Receptors, Androgen, Serum Albumin, Bovine, Dihydrotestosterone, Testosterone, Tumor Cells, Cultured, Signal Transduction, Prostate-Specific Antigen, Phosphatidylinositol 3-Kinases, rac GTP-Binding Proteins, cdc42 GTP-Binding Protein, rac1 GTP-Binding Protein, Focal Adhesion Protein-Tyrosine Kinases, Focal Adhesion Kinase 1

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review