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Regulation by Follicle-Stimulating Hormone of the Synthesis of Aromatase Cytochrome P-450 in Human Granulosa Cells
Open accessAbstract
The effects of FSH to increase the activity of aromatase, as well as the synthesis of the components of the aromatase enzyme complex, have been studied in human ovarian granulosa cells obtained from women undergoing oocyte retrieval. FSH increased aromatase activity, as well as the synthesis of aromatase cytochrome P-450 (P-450AROM) in a time-dependent fashion, whereas in the absence of FSH, both activity and synthesis declined with duration of culture. The effect of FSH was mimicked by forskolin, an activator of adenylate cyclase. FSH also increased the synthesis of NADPH-cytochrome P-450 reductase, but to a relatively modest extent. The levels of hybridizable mRNA species encoding cytochrome P-450AROM of lengths 3.0, 2.4, and 1.6 kilobases were also increased with FSH treatment. It is concluded that the regulation of aromatase activity by FSH in human granulosa cells is mediated primarily by changes in the synthesis of cytochrome P-450AROM, that this action of FSH is mediated by cAMP, and that the changes in cytochrome P-450AROM synthesis are the consequences of changes in the levels of mRNA encoding this enzyme.
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Metadata
- Authors
- Steinkampf, M. P., Mendelson, C. R., & Simpson, E. R.
- Affiliations
- The University of Texas System (US)
- University of Texas Health Science Center at Dallas (US)
- ORCID
- Mendelson, Carole R.
- Venue
- Molecular Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1987-07
- Volume
- 1
- Issue
- 7
- Pages
- 465-471
- ISSN
- 0888-8809 (ISSN-L)
- 1944-9917
- OpenAlex
- W2108477359
- Cited by
- 135
- References
- 27
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2022: 3 · 2021: 1 · 2020: 4 · 2019: 2 · 2018: 3 · 2017: 1 · 2016: 1 · 2015: 2 · 2014: 2 · 2013: 3 · 2012: 2
- FWCI
- 10.38
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Alabama at Birmingham (35294)
- National Institutes of Health (AM-31206)
- U.S. Public Health Service (5 T32)
- National Institute on Aging (AG-00306)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (T32HD007190, 5-T32 HD07190)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Reproductive Biology and Fertility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
- Health Sciences › Medicine › Reproductive Medicine › Ovarian function and disorders
- Keywords
- follicle-stimulating hormone, aromatase, human granulosa cells, enzyme synthesis, cAMP signaling, forskolin, NADPH-cytochrome P450 reductase, mRNA levels, steroidogenesis, oocyte retrieval
- MeSH terms
- Aromatase, Cells, Cultured, Female, Colforsin, Follicle Stimulating Hormone, Granulosa Cells, Humans, NADPH-Ferrihemoprotein Reductase, RNA, Messenger, Blotting, Northern
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