Cited by 190EEG and Brain-Computer InterfacesIon Transport and Channel RegulationCerebrospinal fluid and hydrocephalus

Electroencephalography: Basic Principles, Clinical Applications, and Related Fields, Fifth Edition

Henry, J. Craig·2006·Neurology

Abstract

Highly differentiated human proximal tubule (HPT) cells in primary culture were established from heterogeneous suspension of tubules prepared from the human renal cortex by an original two-step procedure. First, gluconeogenic-competent HPT cells were selected by using a hormonally defined serum-free medium without glucose or insulin; then, the selected HPT cells were grown in a medium containing a low concentration of glucose (1 mM) and insulin (0.5 micrograms/mL) but no antibiotics. HPT cells grown on plastic support formed confluent, cobblestone-like monolayers with numerous mitochondria and pinocytosis vacuoles, solitary cilia, junctional complexes, and a well-developed brush border consisting of densely packed microvilli. Compared with cell monolayers on plastic support, HPT cells grown on porous filter membranes showed better morphologic differentiation. HPT cell monolayers expressed the following differentiated functions of the proximal tubule in situ: a low-affinity, high-capacity Na(+)-dependent glucose transport system inhibited by phlorizin, a high-affinity Na(+)-dependent phosphate transport system, a basolateral organic cation uptake inhibited by mepiperphenidol, parathyroid hormone-sensitive cAMP synthesis, brush-border hydrolase activities, gluconeogenesis-associated enzymes, glutathione-S-transferases and N-acetyl-beta-D-glucosaminidase. The medium containing low glucose and insulin concentrations markedly limited the increase in glycolysis but did not prevent the falls in gluconeogenesis and brush-border hydrolase activity at any time of the culture period. Similar decreases of brush border enzyme activities were obtained for HPT cells grown either on plastic or on porous filter membrane. A thorough characterization study demonstrated that this simple and preparative experimental approach makes it possible to establish highly differentiated HPT cells in primary culture suitable for investigating human renal proximal tubular cell function.

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Crossref · 10.1212/01.wnl.0000243257.85592.9a

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Authors
Henry, J. C.
Corresponding author
Henry, J. Craig
Venue
Neurology
Published
2006-12-12
Volume
67
Issue
11
Pages
2092
ISSN
  • 0028-3878 (ISSN-L)
  • 1526-632X
OpenAlex
W2101640127
Cited by
190
References
0
Citations by year
2026: 18 · 2025: 19 · 2024: 18 · 2023: 25 · 2022: 49 · 2021: 24 · 2020: 12 · 2019: 5 · 2018: 3 · 2017: 1 · 2016: 3 · 2015: 2 · 2013: 2 · 2012: 1
FWCI
0.64
Type
journal article
Language
en
Open access
closed
Locations
Neurology (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Cognitive Neuroscience › EEG and Brain-Computer Interfaces
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Cerebrospinal fluid and hydrocephalus
Keywords
EEG, EEG signal processing

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