Nemo is an inducible antagonist of Wingless signaling during Drosophila wing development
Open accessAbstract
The cellular events that govern patterning during animal development must be precisely regulated. This is achieved by extrinsic factors and through the action of both positive and negative feedback loops. Wnt/Wg signals are crucial across species in many developmental patterning events. We report that Drosophila nemo (nmo) acts as an intracellular feedback inhibitor of Wingless (Wg) and that it is a novel Wg target gene. Nemo antagonizes the activity of the Wg signal, as evidenced by the finding that reduction of nmo rescues the phenotypic defects induced by misexpression of various Wg pathway components. In addition, the activation of Wg-dependent gene expression is suppressed in wing discs ectopically expressing nmo and enhanced cell autonomously in nmo mutant clones. We find that nmo itself is a target of Wg signaling in the imaginal wing disc. nmo expression is induced upon high levels of Wg signaling and can be inhibited by interfering with Wg signaling. Finally, we observe alterations in Arm stabilization upon modulation of Nemo. These observations suggest that the patterning mechanism governed by Wg involves a negative feedback circuit in which Wg induces expression of its own antagonist Nemo.
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Nmo could be a suppressor of Wnt signaling.
“Subsequent Drosophila studies also indicated that Nmo could be a suppressor of Wnt signaling18)–20)”
From Mammalian Nemo-like kinase enhances .BETA.-catenin-TCF transcription activity in human osteosarcoma and neuroblastoma cells (2007) · cites this paper as [20]
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Ishitani, T., Ninomiya-Tsuji, J., Nagai, S.-I., Nishita, M., Meneghini, M., Barker, N., Waterman, M., Bowerman, B., Clevers, H., Shibuya, H., et al. (1999) · Nature · doi:10.1038/21674 · Cited by 624 · see citing works
Rotation of photoreceptor clusters in the developing drosophila eye requires the nemo gene
Choi, K.-W., & Benzer, S. (1994) · Cell · doi:10.1016/0092-8674(94)90579-7 · Cited by 339 · see citing works
Ishitani, T., Ninomiya-Tsuji, J., & Matsumoto, K. (2003) · Molecular and Cellular Biology · doi:10.1128/mcb.23.4.1379-1389.2003 · Cited by 212 · see citing works
A <i>Drosophila Axin</i> homolog, <i>Daxin</i>, inhibits Wnt signaling
Willert, K., Logan, C. Y., Arora, A., Fish, M., & Nusse, R. (1999) · Development · doi:10.1242/dev.126.18.4165 · Cited by 117 · see citing works
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Research works printed but not linked: 2
- Brott, B., Pinsky, B. and Reikson, R. (1998). Nlk is a murine protein kinase related to Erk/MAP kinases and localized in the nucleus. Proc. Natl. Acad. Sci. USA 95, 963-968.
- Schweizer, L., Nellen, D. and Basler, K. (2003). Requirement for Pangolin/dTCF in Drosophila Wingless signaling. Proc. Natl. Acad. Sci. USA 100, 5846-5851.
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Wnt signaling arrests effector T cell differentiation and generates CD8+ memory stem cells
Gattinoni, L., Zhong, X.-S., Palmer, D. C., Ji, Y., Hinrichs, C. S., Yu, Z., Wrzesinski, C., Boni, A., Cassard, L., Garvin, L. M., et al. (2009) · Nature Medicine · doi:10.1038/nm.1982 · Cited by 1,031 · see citing works
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- Authors
- Zeng, Y. A., & Verheyen, E. M.
- Affiliations
- Simon Fraser University (CA)
- Corresponding author
- Verheyen, Esther M.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2004-06-15
- Volume
- 131
- Issue
- 12
- Pages
- 2911-2920
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W1997180986
- Cited by
- 60
- References
- 4
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 3 · 2016: 1 · 2015: 4 · 2014: 3 · 2013: 3 · 2012: 5
- FWCI
- 1.30
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Development (publishedVersion)
- PubMed (publishedVersion)
- Rare & Special e-Zone (The Hong Kong University of Science and Technology) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Hedgehog Signaling Pathway Studies
- Keywords
- NEMO, Wingless signaling, negative feedback loop, wing development, wing imaginal disc, Drosophila, developmental patterning, gene expression regulation
- MeSH terms
- Animals, Cloning, Molecular, Drosophila melanogaster, Embryo, Nonmammalian, Feedback, Morphogenesis, Proteins, Proto-Oncogene Proteins, Wings, Animal, In Situ Hybridization, Gene Expression Regulation, Developmental, Body Patterning, Mitogen-Activated Protein Kinases, Drosophila Proteins, Wnt1 Protein
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