A Drosophila Axin homolog, Daxin, inhibits Wnt signaling
Abstract
The vertebrate Axin protein, the product of the mouse fused gene, binds to β-catenin to inhibit Wnt signaling. We have identified a homolog of Axin in Drosophila, Daxin. Using double-stranded RNA interference, we generated loss-of-function phenotypes that are similar to overexpression of the Drosophila Wnt gene wingless (wg). Overexpression of Daxin produces phenotypes similar to loss of wg. In addition, we show that Daxin overexpression can modify phenotypes elicited by wg and another Drosophila Wnt gene, DWnt-2. Using immunoprecipitation of endogenous Daxin protein from embryos we show that Daxin interacts with Armadillo and Zeste-white 3. The loss-of-function and overexpression phenotypes show that Daxin, like its mammalian counterpart, acts as a negative regulator of wg/Wnt signaling.
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Wnt stimulation regulates the stability of Axin, with a de-phosphorylation of Axin and a decrease in cytoplasmic levels of Axin documented.
“It is important to note that Wnt stimulation also regulates the stability of Axin for with Wnt stimulation a de-phosphorylation of Axin and a decrease in cytoplasmic levels of Axin has been documented.29–31”
From Wnt signal transduction pathways (2008) · cites this paper as [30]
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- Authors
- Willert, K., Logan, C. Y., Arora, A., Fish, M., & Nusse, R.
- Affiliations
- Howard Hughes Medical Institute (US)
- Howard Hughes Medical Institute and Department of Developmental Biology, Stanford University School of Medicine , Stanford, CA 94305, USA
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 1999-09-15
- Volume
- 126
- Issue
- 18
- Pages
- 4165-4173
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2168820955
- Cited by
- 117
- References
- 48
- Citations by year
- 2026: 1 · 2025: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 3 · 2017: 3 · 2016: 2 · 2015: 6 · 2014: 2 · 2013: 3 · 2012: 3
- FWCI
- 4.34
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Development (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Hedgehog Signaling Pathway Studies
- Keywords
- Wnt signaling, wingless, β-catenin, armadillos, Drosophila, loss-of-function phenotypes
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Carrier Proteins, Drosophila melanogaster, Embryo, Nonmammalian, Eye, Eye Abnormalities, Female, Genetic Techniques, Molecular Sequence Data, Ovary, Proteins, Proto-Oncogene Proteins, Repressor Proteins, RNA, Transcription Factors, Wings, Animal, Signal Transduction, Trans-Activators, Protein Serine-Threonine Kinases, Sequence Homology, Amino Acid, Gene Expression Regulation, Developmental, Insect Proteins, Expressed Sequence Tags, Drosophila Proteins, Glycogen Synthase Kinase 3, Adaptor Proteins, Signal Transducing, Wnt2 Protein, Wnt1 Protein, Armadillo Domain Proteins, Axin Protein
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