The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Inducible expression of an hsp68-lacZ hybrid gene in transgenic mice
Abstract
Transgenic mice have been generated that express the E. coli β-galactosidase gene under the control of the promoter from the mouse heat-shock gene, hsp68. Sequences from -664 to +113 relative to the start of transcription of the hsp68 gene were sufficient to direct stress-induced expression of the β-galactosidase gene in adult tail tissue and various tissues of fetal stages of development. Expression was detected in situ by staining with the chromogenic substrate, X-gal. The hybrid gene was refractory to induction in preimplantation embryos until the blastocyst stage of development, as reported for the endogenous hsp68 gene. No constitutive expression was observed by in situ staining or Northern analysis at any stage of development, even in tissues that constitutively express the endogenous hsp68 gene. We conclude that the hsp68 promoter region included in the construct contains sufficient sequence information for heat and arsenite inducibility, but it does not contain sequences controlling tissue-specific expression during development. This tightly regulated inducible promoter may provide a useful tool for short-term inducible gene expression in transgenic mice.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
Feinberg, A. P., & Vogelstein, B. (1983) · Analytical Biochemistry · doi:10.1016/0003-2697(83)90418-9 · Cited by 24,887
Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles
Kozak, M. (1983) · Microbiological Reviews · doi:10.1128/mmbr.47.1.1-45.1983 · Cited by 1,502
Munro, S., & Pelham, H. R. B. (1986) · Cell · doi:10.1016/0092-8674(86)90746-4 · Cited by 1,449
Use of a recombinant retrovirus to study post-implantation cell lineage in mouse embryos.
Sanes, J. R., Rubenstein, J. L., & Nicolas, J. F. (1986) · The EMBO Journal · doi:10.1002/j.1460-2075.1986.tb04620.x · Cited by 1,105
A regulatory upstream promoter element in the Drosophila Hsp 70 heat-shock gene
Pelham, H. R. B. (1982) · Cell · doi:10.1016/0092-8674(82)90249-5 · Cited by 1,000
Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer.
Price, J., Turner, D., & Cepko, C. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.1.156 · Cited by 873
Immunoglobulin heavy chain binding protein
Haas, I. G., & Wabl, M. (1983) · Nature · doi:10.1038/306387a0 · Cited by 845
Uncoating ATPase is a member of the 70 kilodalton family of stress proteins
Chappell, T. G., Welch, W. J., Schlossman, D. M., Palter, K. B., Schlesinger, M. J., & Rothman, J. E. (1986) · Cell · doi:10.1016/0092-8674(86)90532-5 · Cited by 592
A drosophila RNA polymerase II transcription factor binds to the regulatory site of an hsp 70 gene
Parker, C. S., & Topol, J. (1984) · Cell · doi:10.1016/0092-8674(84)90323-4 · Cited by 499
Activating protein factor binds in vitro to upstream control sequences in heat shock gene chromatin
Wu, C. (1984) · Nature · doi:10.1038/311081a0 · Cited by 306
Cited by
The Endothelial-Specific MicroRNA miR-126 Governs Vascular Integrity and Angiogenesis
Wang, S., Aurora, A. B., Johnson, B. A., Qi, X., McAnally, J., Hill, J. A., Richardson, J. A., Bassel-Duby, R., & Olson, E. N. (2008) · Developmental Cell · doi:10.1016/j.devcel.2008.07.002 · Cited by 1,617
VISTA Enhancer Browser—a database of tissue-specific human enhancers
Visel, A., Minovitsky, S., Dubchak, I., & Pennacchio, L. A. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkl822 · Cited by 1,080
In vivo enhancer analysis of human conserved non-coding sequences
Pennacchio, L. A., Ahituv, N., Moses, A. M., Prabhakar, S., Nobrega, M. A., Shoukry, M., Minovitsky, S., Dubchak, I., Holt, A., Lewis, K. D., et al. (2006) · Nature · doi:10.1038/nature05295 · Cited by 1,165
Enhancer redundancy provides phenotypic robustness in mammalian development
Osterwalder, M., Barozzi, I., Tissières, V., Fukuda-Yuzawa, Y., Mannion, B. J., Afzal, S. Y., Lee, E. A., Zhu, Y., Plajzer-Frick, I., Pickle, C. S., et al. (2018) · Nature · doi:10.1038/nature25461 · Cited by 642
Genomic views of distant-acting enhancers
Visel, A., Rubin, E. M., & Pennacchio, L. A. (2009) · Nature · doi:10.1038/nature08451 · Cited by 610
Metadata
- Authors
- Kothary, R., Clapoff, S., Darling, S., Perry, M. D., Moran, L. A., & Rossant, J.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 1989-04-01
- Volume
- 105
- Issue
- 4
- Pages
- 707-714
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2106384420
- Cited by
- 323
- References
- 23
- FWCI
- 2.73
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Inducible expression of an hsp68-lacZ hybrid gene in transgenic mice. https://app.cochat.ai/papers/10.1242/dev.105.4.707
- The paper
- Kothary, R., Clapoff, S., Darling, S., Perry, M. D., Moran, L. A., & Rossant, J. (1989). Inducible expression of an hsp68-lacZ hybrid gene in transgenic mice. Development. https://doi.org/10.1242/dev.105.4.707
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
