Mouse oocytes injected with testicular spermatozoa or round spermatids can develop into normal offspring

Abstract

Genomic imprinting occurs in both male and female gametes during gametogenesis, but the exact time when imprinting begins and ends is unknown. In the present study we injected nuclei of testicular spermatozoa and round spermatids into mature mouse oocytes to see whether these nuclei are able to participate in syngamy and normal embryonic development. If the injected oocytes develop into normal fertile offspring, imprinting in the male germ cells used must have been completed by the time of injection. Ninety-two percent of mouse oocytes injected with testicular spermatozoa survived and 94% of these were fertilized normally (extrusion of the second polar body and formation of male and female pronuclei). When 44 two-cell embryos so created were transferred to 5 foster mothers, 24 (54.5%) developed into normal offspring. Unlike testicular spermatozoa, round spermatids could not activate the oocytes, and therefore the oocytes had to be activated artificially either before or after spermatid injection. The highest rate (77%) of normal fertilization was obtained when the oocytes were first activated by electric current, then injected individually with a single spermatid nucleus. When 131 two-cell embryos were transferred to 15 foster mothers, 37 (28.2%) reached full term. All but two grew into healthy adults. Thus, it would appear that gametic imprinting in mouse spermatogenic cells is completed before spermiogenesis begins. Under the experimental conditions employed, spermatid nuclei were less efficient than testicular sperm nuclei in producing normal offspring, but perhaps this was due to technical rather than inherent problems.