The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Selective reduction of dormant maternal mRNAs in mouse oocytes by RNA interference
Abstract
Specific mRNA degradation mediated by double-stranded RNA (dsRNA), which is termed RNA interference (RNAi), is a useful tool with which to study gene function in several systems. We report here that in mouse oocytes, RNAi provides a suitable and robust approach to study the function of dormant maternal mRNAs. Mos (originally known as c-mos) and tissue plasminogen activator (tPA, Plat) mRNAs are dormant maternal mRNAs that are recruited during oocyte maturation; translation of Mos mRNA results in the activation of MAP kinase. dsRNA directed towards Mos or Plat mRNAs in mouse oocytes effectively results in the specific reduction of the targeted mRNA in both a time- and concentration-dependent manner. Moreover, dsRNA is more potent than either sense or antisense RNAs. Targeting the Mos mRNA results in inhibiting the appearance of MAP kinase activity and can result in parthenogenetic activation. Mos dsRNA, therefore, faithfully phenocopies the Mos null mutant. Targeting the Plat mRNA with Plat dsRNA results in inhibiting production of tPA activity. Finally, effective reduction of the Mos and Plat mRNA is observed with stoichiometric amounts of Mos and Plat dsRNA, respectively.
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Cites
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429
Cited by
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., & Tuschl, T. (2001) · Nature · doi:10.1038/35078107 · Cited by 9,456
RNA interference is mediated by 21- and 22-nucleotide RNAs
Elbashir, S. M., Lendeckel, W., & Tuschl, T. (2001) · Genes & Development · doi:10.1101/gad.862301 · Cited by 3,310
Caplen, N. J., Parrish, S., Imani, F., Fire, A., & Morgan, R. A. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.171251798 · Cited by 1,043
siRNAs: applications in functional genomics and potential as therapeutics
Dorsett, Y., & Tuschl, T. (2004) · Nature Reviews Drug Discovery · doi:10.1038/nrd1345 · Cited by 909
siRNA-mediated gene silencing in vitro and in vivo
Xia, H., Mao, Q., Paulson, H. L., & Davidson, B. L. (2002) · Nature Biotechnology · doi:10.1038/nbt739 · Cited by 873
Metadata
- Authors
- Svoboda, P., Stein, P., Hayashi, H., & Schultz, R. M.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2000-10-01
- Volume
- 127
- Issue
- 19
- Pages
- 4147-4156
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2150809319
- Cited by
- 407
- References
- 1
- FWCI
- 41.66
- Type
- journal article
- Language
- en
- Open access
- closed
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- Svoboda, P., Stein, P., Hayashi, H., & Schultz, R. M. (2000). Selective reduction of dormant maternal mRNAs in mouse oocytes by RNA interference. Development. https://doi.org/10.1242/dev.127.19.4147
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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