The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
A stepwise model of Reaction-Diffusion and Positional-Information governs self-organized human peri-gastrulation-like patterning
Open accessAbstract
How position-dependent cell fate acquisition occurs during embryogenesis is a central question in developmental biology. To study this process, we developed a defined, high-throughput assay to induce peri-gastrulation-associated patterning in geometrically-confined human pluripotent stem cell (hPSC) colonies. We observed that, upon BMP4 treatment, phosphorylated SMAD1 (pSMAD1) activity in the colonies organized into a radial gradient. We developed a Reaction-Diffusion (RD) based computational model and observed that the self-organization of pSMAD1 signaling was consistent with the RD principle. Consequent fate acquisition occurred as a function of both pSMAD1 signaling strength and duration of induction – consistent with the Positional-Information (PI) paradigm. We propose that the self-organized peri-gastrulation-like fate patterning in BMP4 treated geometrically-confined hPSC colonies arises via a stepwise model of RD, and PI. This two-step model predicted experimental responses to perturbations of key parameters such as colony size, and BMP4 dose. Furthermore, it also predicted experimental conditions that resulted in RD-like periodic patterning in large hPSC colonies, and rescued peri-gastrulation-like patterning in colony sizes previously thought to be reticent to this behaviour.
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Cites
The chemical basis of morphogenesis
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Reaction-Diffusion Model as a Framework for Understanding Biological Pattern Formation
Kondo, S., & Miura, T. (2010) · Science · doi:10.1126/science.1179047 · Cited by 1,651
A method to recapitulate early embryonic spatial patterning in human embryonic stem cells
Warmflash, A., Sorre, B., Etoc, F., Siggia, E. D., & Brivanlou, A. H. (2014) · Nature Methods · doi:10.1038/nmeth.3016 · Cited by 885
The chemical basis of morphogenesis
Turing, A. M. (1990) · Bulletin of Mathematical Biology · doi:10.1007/bf02459572 · Cited by 434
Conserved Patterns of Cell Movements during Vertebrate Gastrulation
Solnica-Krezel, L. (2005) · Current Biology · doi:10.1016/j.cub.2005.03.016 · Cited by 391
Self-organizing human cardiac microchambers mediated by geometric confinement
Ma, Z., Wang, J., Loskill, P., Huebsch, N., Koo, S., Svedlund, F. L., Marks, N. C., Hua, E. W., Grigoropoulos, C. P., Conklin, B. R., et al. (2015) · Nature Communications · doi:10.1038/ncomms8413 · Cited by 214
Cited by
Progress and potential in organoid research
Rossi, G., Manfrin, A., & Lutolf, M. P. (2018) · Nature Reviews Genetics · doi:10.1038/s41576-018-0051-9 · Cited by 1,182
Engineering Stem Cell Self-organization to Build Better Organoids
Brassard, J. A., & Lutolf, M. P. (2019) · Cell Stem Cell · doi:10.1016/j.stem.2019.05.005 · Cited by 373
Kim, Y., Jang, H., Seo, K., Kim, J. H., Lee, B., Cho, H. M., Kim, H. J., Yang, E., Kim, H., Gim, J.-A., et al. (2022) · Stem Cell Reports · doi:10.1016/j.stemcr.2021.11.005 · Cited by 33
Metadata
- Authors
- Tewary, M., Ostblom, J., Prochazka, L., Zulueta-Coarasa, T., Shakiba, N., Fernandez-Gonzalez, R., & Zandstra, P. W.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2017-01-01
- Volume
- 144
- Issue
- 23
- Pages
- 4298-4312
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2751928832
- Cited by
- 158
- References
- 7
- FWCI
- 6.89
- Type
- journal article
- Language
- en
- Open access
- hybrid
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