The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
BMP-9 signals via ALK1 and inhibits bFGF-induced endothelial cell proliferation and VEGF-stimulated angiogenesis
Abstract
Genetic studies in mice and humans have shown that the transforming growth factor-β (TGF-β) type-I receptor activin receptor-like kinase 1 (ALK1) and its co-receptor endoglin play an important role in vascular development and angiogenesis. Here, we demonstrate that ALK1 is a signalling receptor for bone morphogenetic protein-9 (BMP-9) in endothelial cells (ECs). BMP-9 bound with high affinity to ALK1 and endoglin, and weakly to the type-I receptor ALK2 and to the BMP type-II receptor (BMPR-II) and activin type-II receptor (ActR-II) in transfected COS cells. Binding of BMP-9 to ALK2 was greatly facilitated when BMPR-II or ActR-II were co-expressed. Whereas BMP-9 predominantly bound to ALK1 and BMPR-II in ECs, it bound to ALK2 and BMPR-II in myoblasts. In addition, we observed binding of BMP-9 to ALK1 and endoglin in glioblastoma cells. BMP-9 activated Smad1 and/or Smad5, and induced ID1 protein and endoglin mRNA expression in ECs. Furthermore, BMP-9 was found to inhibit basic fibroblast growth factor (bFGF)-stimulated proliferation and migration of bovine aortic ECs (BAECs) and to block vascular endothelial growth factor (VEGF)-induced angiogenesis. Taken together, these results suggest that BMP-9 is a physiological ALK1 ligand that plays an important role in the regulation of angiogenesis.
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Cites
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Self-citationDefective Angiogenesis in Mice Lacking Endoglin
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Cited by
Bone morphogenetic proteins: a critical review
Bragdon, B., Moseychuk, O., Saldanha, S., King, D., Julian, J., & Nohe, A. (2011) · Cellular Signalling · doi:10.1016/j.cellsig.2010.10.003 · Cited by 691
TGF-β signaling in vascular biology and dysfunction
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Self-citationHereditary haemorrhagic telangiectasia: Pathophysiology, diagnosis and treatment
Shovlin, C. L. (2010) · Blood Reviews · doi:10.1016/j.blre.2010.07.001 · Cited by 502
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Bone Morphogenetic Protein-9 Is a Circulating Vascular Quiescence Factor
David, L., Mallet, C., Keramidas, M., Lamandé, N., Gasc, J.-M., Dupuis-Girod, S., Plauchu, H., Feige, J.-J., & Bailly, S. (2008) · Circulation Research · doi:10.1161/circresaha.107.165530 · Cited by 416
Metadata
- Authors
- Scharpfenecker, M., van Dinther, M., Liu, Z., van Bezooijen, R. L., Zhao, Q., Pukac, L., Löwik, C. W. G. M., & ten Dijke, P.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 2007-03-15
- Volume
- 120
- Issue
- 6
- Pages
- 964-972
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2158847377
- Cited by
- 531
- References
- 47
- FWCI
- 12.33
- Type
- journal article
- Language
- en
- Open access
- closed
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- Scharpfenecker, M., van Dinther, M., Liu, Z., van Bezooijen, R. L., Zhao, Q., Pukac, L., Löwik, C. W. G. M., & ten Dijke, P. (2007). BMP-9 signals via ALK1 and inhibits bFGF-induced endothelial cell proliferation and VEGF-stimulated angiogenesis. Journal of Cell Science. https://doi.org/10.1242/jcs.002949
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