The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Oxygen-regulated expression of the RNA-binding proteins RBM3 and CIRP by a HIF-1-independent mechanism
Abstract
The transcriptional regulation of several dozen genes in response to low oxygen tension is mediated by hypoxia-inducible factor 1 (HIF-1), a heterodimeric protein composed of two subunits, HIF-1α and HIF-1β. In the HIF-1α-deficient human leukemic cell line, Z-33, exposed to mild (8% O2) or severe (1% O2) hypoxia, we found significant upregulation of two related heterogenous nuclear ribonucleoproteins, RNA-binding motif protein 3 (RBM3) and cold inducible RNA-binding protein (CIRP), which are highly conserved cold stress proteins with RNA-binding properties. Hypoxia also induced upregulation of RBM3 and CIRP in the murine HIF-1β-deficient cell line, Hepa-1 c4. In various HIF-1 competent cells, RBM3 and CIRP were induced by moderate hypothermia (32°C) but hypothermia was ineffective in increasing HIF-1α or vascular endothelial growth factor (VEGF), a known HIF-1 target. In contrast, iron chelators induced VEGF but not RBM3 or CIRP. The RBM3 and CIRP mRNA increase after hypoxia was inhibited by actinomycin-D, and in vitro nuclear run-on assays demonstrated specific increases in RBM3 and CIRP mRNA after hypoxia, which suggests that regulation takes place at the level of gene transcription. Hypoxia-induced RBM3 or CIRP transcription was inhibited by the respiratory chain inhibitors NaN3 and cyanide in a dose-dependent fashion. However, cells depleted of mitochondria were still able to upregulate RBM3 and CIRP in response to hypoxia. Thus, RBM3 and CIRP are adaptatively expressed in response to hypoxia by a mechanism that involves neither HIF-1 nor mitochondria.
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Cites
Wang, G. L., Jiang, B. H., Rue, E. A., & Semenza, G. L. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.12.5510 · Cited by 6,212
The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis
Maxwell, P. H., Wiesener, M. S., Chang, G.-W., Clifford, S. C., Vaux, E. C., Cockman, M. E., Wykoff, C. C., Pugh, C. W., Maher, E. R., & Ratcliffe, P. J. (1999) · Nature · doi:10.1038/20459 · Cited by 5,180
Hypoxia — a key regulatory factor in tumour growth
Harris, A. L. (2002) · Nature Reviews Cancer · doi:10.1038/nrc704 · Cited by 5,177
Activation of vascular endothelial growth factor gene transcription by hypoxia-inducible factor 1
Forsythe, J. A., Jiang, B.-H., Iyer, N. V., Agani, F., Leung, S. W., Koos, R. D., & Semenza, G. L. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.9.4604 · Cited by 3,981
Sowter, H., Ratcliffe, P. J., Watson, P. H., Greenberg, A. H., & Harris, A. L. (2001) · PubMed · Cited by 706
Wiesener, M. S., Turley, H., Allen, W. E., Willam, C., Eckardt, K.-U., Talks, K. L., Wood, S. M., Gatter, K. C., Harris, A. L., Pugh, C. W., et al. (1998) · Blood · doi:10.1182/blood.v92.7.2260 · Cited by 635
Invited Review: Effects of heat and cold stress on mammalian gene expression
Sonna, L. A., Fujita, J., Gaffin, S. L., & Lilly, C. M. (2002) · Journal of Applied Physiology · doi:10.1152/japplphysiol.01143.2001 · Cited by 628
Self-citationIdentification of a Novel Hypoxia-Inducible Factor 1-Responsive Gene, RTP801 , Involved in Apoptosis
Shoshani, T., Faerman, A., Mett, I., Zelin, E., Tenne, T., Gorodin, S., Moshel, Y., Elbaz, S., Budanov, A., Chajut, A., et al. (2002) · Molecular and Cellular Biology · doi:10.1128/mcb.22.7.2283-2293.2002 · Cited by 546
Maxwell, P. H., Pugh, C. W., & Ratcliffe, P. J. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.6.2423 · Cited by 411
A Glycine-rich RNA-binding Protein Mediating Cold-inducible Suppression of Mammalian Cell Growth
Nishiyama, H., Itoh, K., Kaneko, Y., Kishishita, M., Yoshida, O., & Fujita, J. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.137.4.899 · Cited by 376
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A Tissue-Mapped Axolotl De Novo Transcriptome Enables Identification of Limb Regeneration Factors
Bryant, D. M., Johnson, K., DiTommaso, T., Tickle, T., Couger, M. B., Payzin-Dogru, D., Lee, T. J., Leigh, N. D., Kuo, T.-H., Davis, F. G., et al. (2017) · Cell Reports · doi:10.1016/j.celrep.2016.12.063 · Cited by 979
Kornmann, B., Schaad, O., Bujard, H., Takahashi, J. S., & Schibler, U. (2007) · PLoS Biology · doi:10.1371/journal.pbio.0050034 · Cited by 658
Denning, N.-L., Aziz, M., Gurien, S. D., & Wang, P. (2019) · Frontiers in Immunology · doi:10.3389/fimmu.2019.02536 · Cited by 623
Qiang, X., Yang, W.-L., Wu, R., Zhou, M., Jacob, A., Dong, W., Kuncewitch, M., Ji, Y., Yang, H., Wang, H., et al. (2013) · Nature Medicine · doi:10.1038/nm.3368 · Cited by 443
Self-citationLiao, S., Zhao, X., Han, Y., Zhang, J., Wang, L., Xia, L., Zhao, K., Zheng, Y., Guo, M., & Chen, G. (2009) · PROTEOMICS · doi:10.1002/pmic.200800922 · Cited by 64
Metadata
- Authors
- Wellmann, S., Bührer, C., Moderegger, E., Zelmer, A., Kirschner, R., Koehne, P., Fujita, J., & Seeger, K.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 2004-04-01
- Volume
- 117
- Issue
- 9
- Pages
- 1785-1794
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2096048497
- Cited by
- 256
- References
- 31
- FWCI
- 2.08
- Type
- journal article
- Language
- en
- Open access
- closed
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- Wellmann, S., Bührer, C., Moderegger, E., Zelmer, A., Kirschner, R., Koehne, P., Fujita, J., & Seeger, K. (2004). Oxygen-regulated expression of the RNA-binding proteins RBM3 and CIRP by a HIF-1-independent mechanism. Journal of Cell Science. https://doi.org/10.1242/jcs.01026
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