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Fibroblast growth factor 2 endocytosis in endothelial cells proceed via syndecan-4-dependent activation of Rac1 and a Cdc42-dependent macropinocytic pathway

Tkachenko, Eugene, Lutgens, Esther, Stan, Radu-Virgil, Simons, Michael·2004·Journal of Cell Science
DOI 10.1242/jcs.01190
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Abstract

Full activity of fibroblast growth factors (FGFs) requires their internalization in addition to the interaction with cell surface receptors. Recent studies have suggested that the transmembrane proteoglycan syndecan-4 functions as a FGF2 receptor. In this study we investigated the molecular basis of syndecan endocytosis and its role in FGF2 internalization in endothelial cells. We found that syndecan-4 uptake, induced either by treatment with FGF2 or by antibody clustering, requires the integrity of plasma membrane lipid rafts for its initiation, occurs in a non-clathrin-, non-dynamin-dependent manner and involves Rac1, which is activated by syndecan-4 clustering. FGF2 was internalized in a complex with syndecan-4 in 70 kDa dextran-containing endocytic vesicles. FGF2 and syndecan-4 but not dextran endocytosis were blocked by the dominant negative Rac1 while amiloride and the dominant-negative Cdc42 blocked internalization of dextran in addition to FGF2 and syndecan-4. Taken together, these results demonstrate that FGF2 endocytosis requires syndecan-4 clustering-dependent activation of Rac1 and the intact CDC42-dependent macropinocytic pathway.

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  • Endocytosis of syndecans occurs in a clathrin-independent manner with relatively slow kinetics compared with clathrin-mediated uptake of ligands such as lipoproteins by LDLRs.

    “In cells that have been studied, endocytosis of syndecans occurs in a clathrin-independent manner with relatively slow kinetics (t1/2 = 20–60 minutes) (15, 21, 58, 59) compared with clathrin-mediated uptake of ligands, e.g., uptake of lipoproteins by LDLRs (t1/2 = 5–10 minutes) (60).”

    From Syndecan-1 is the primary heparan sulfate proteoglycan mediating hepatic clearance of triglyceride-rich lipoproteins in mice (2009) · cites this paper as [59]

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Research works printed but not linked: 41

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Metadata

Authors
Tkachenko, E., Lutgens, E., Stan, R.-V., & Simons, M.
Affiliations
  • Angiogenesis Research Center and Section of Cardiology, Department of Medicine and Department of Pharmacology and Toxicology, Dartmouth Medical School, One Medical Center Drive, Lebanon, NH 03756, USA
  • Department of Pathology, Dartmouth Medical School, One Medical Center Drive, Lebanon, NH 03756, USA
Venue
Journal of Cell Science
Publisher
The Company of Biologists
Published
2004-07-01
Volume
117
Issue
15
Pages
3189-3199
ISSN
  • 1477-9137
  • 0021-9533
DOI
10.1242/jcs.01190
Cited by
141
References
0
Language
en

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