The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Role for Rab7 in maturation of late autophagic vacuoles
Open accessAbstract
The small GTP binding protein Rab7 has a role in the late endocytic pathway and lysosome biogenesis. The role of mammalian Rab7 in autophagy is, however, unknown. We have addressed this by inhibiting Rab7 function with RNA interference and overexpression of dominant negative Rab7. We show here that Rab7 was needed for the formation of preferably perinuclear, large aggregates, where the autophagosome marker LC3 colocalised with Rab7 and late endosomal and lysosomal markers. By electron microscopy we showed that these large aggregates corresponded to autophagic vacuoles surrounding late endosomal or lysosomal vesicles. Our experiments with quantitative electron microscopy showed that Rab7 was not needed for the initial maturation of early autophagosomes to late autophagic vacuoles, but that it participated in the final maturation of late autophagic vacuoles. Finally, we showed that the recruitment of Rab7 to autophagic vacuoles was retarded in cells deficient in the lysosomal membrane proteins Lamp1 and Lamp2, which we have recently shown to accumulate late autophagic vacuoles during starvation. In conclusion, our results showed a role for Rab7 in the final maturation of late autophagic vacuoles.
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Citations
Cites
LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing.
Kabeya, Y., Mizushima, N., Ueno, T., Yamamoto, A., Kirisako, T., Noda, T., Kominami, E., Ohsumi, Y., & Yoshimori, T. (2000) · The EMBO Journal · doi:10.1093/emboj/19.21.5720 · Cited by 6,605
Self-citationInduction of autophagy and inhibition of tumorigenesis by beclin 1
Liang, X. H., Jackson, S., Seaman, M., Brown, K., Kempkes, B., Hibshoosh, H., & Levine, B. (1999) · Nature · doi:10.1038/45257 · Cited by 3,347
Formation Process of Autophagosome Is Traced with Apg8/Aut7p in Yeast
Kirisako, T., Baba, M., Ishihara, N., Miyazawa, K., Ohsumi, M., Yoshimori, T., Noda, T., & Ohsumi, Y. (1999) · The Journal of Cell Biology · doi:10.1083/jcb.147.2.435 · Cited by 881 · cited 3×
“This is different from the yeast, where deletion of Ypt7 as good as completely blocked fusion of autophagosomes with the vacuole (Kirisako et al., 1999).”
BackgroundInfluentialIsolation and Characterization of Rat Liver Amphisomes
Berg, T. O., Fengsrud, M., Strømhaug, P. E., Berg, T., & Seglen, P. O. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.34.21883 · Cited by 385 · cited 1×
“Autophagosomes or autophagic vacuoles have been reported to fuse with early (Liou et al., 1997; Tooze et al., 1990) and late endosomes (Berg et al., 1998; Lucocq and Walker, 1997; Punnonen et al., 1993) as well as lysosomes (Dunn, 1990; Gordon et al., 1992; Lawrence and Brown, 1992).”
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Cited by
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Acevedo Arozena, A., Adachi, H., Adams, C. M., Adams, P. D., Adeli, K., et al. (2016) · Autophagy · doi:10.1080/15548627.2015.1100356 · Cited by 5,894
Self-citationRegulation mechanisms and signaling pathways of autophagy
He, C., & Klionsky, D. J. (2009) · Annual Review of Genetics · doi:10.1146/annurev-genet-102808-114910 · Cited by 3,609
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
Klionsky, D. J., Abdel-Aziz, A. K., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Abeliovich, H., Abildgaard, M. H., Abudu, Y. P., Acevedo-Arozena, A., et al. (2021) · Autophagy · doi:10.1080/15548627.2020.1797280 · Cited by 2,897
Self-citationAn Overview of Autophagy: Morphology, Mechanism, and Regulation
Parzych, K. R., & Klionsky, D. J. (2014) · Antioxidants & Redox Signaling · doi:10.1089/ars.2013.5371 · Cited by 2,521
Kimura, S., Noda, T., & Yoshimori, T. (2007) · Autophagy · doi:10.4161/auto.4451 · Cited by 2,278
Metadata
- Authors
- Jäger, S., Bucci, C., Tanida, I., Ueno, T., Kominami, E., Saftig, P., & Eskelinen, E.-L.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 2004-09-15
- Volume
- 117
- Issue
- 20
- Pages
- 4837-4848
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2142044474
- Cited by
- 905
- References
- 4
- FWCI
- 10.58
- Type
- journal article
- Language
- en
- Open access
- green
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- Jäger, S., Bucci, C., Tanida, I., Ueno, T., Kominami, E., Saftig, P., & Eskelinen, E.-L. (2004). Role for Rab7 in maturation of late autophagic vacuoles. Journal of Cell Science. https://doi.org/10.1242/jcs.01370
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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