The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
Homo- and heteromeric assembly of TRPV channel subunits
Abstract
The vanilloid receptor-related TRP channels (TRPV1-6) mediate thermosensation, pain perception and epithelial Ca2+ entry. As the specificity of TRPV channel heteromerization and determinants governing the assembly of TRPV subunits were largely elusive, we investigated the TRPV homo- and heteromultimerization. To analyze the assembly of TRPV subunits in living cells, we generated fluorescent fusion proteins or FLAG-tagged TRPV channel subunits. The interaction between TRPV subunits was assessed by analysis of the subcellular colocalization, fluorescence resonance energy transfer and coimmunoprecipitation. Our results demonstrate that TRPV channel subunits do not combine arbitrarily. With the exception of TRPV5 and TRPV6, TRPV channel subunits preferentially assemble into homomeric complexes. Truncation of TRPV1, expression of cytosolic termini of TRPV1 or TRPV4 and construction of chimeric TRPV channel subunits revealed that the specificity and the affinity of the subunit interaction is synergistically provided by interaction modules located in the transmembrane domains and in the cytosolic termini. The relative contribution of intramolecularly linked interaction modules presumably controls the overall affinity and the specificity of TRPV channel assembly.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The capsaicin receptor: a heat-activated ion channel in the pain pathway
Caterina, M. J., Schumacher, M. A., Tominaga, M., Rosen, T. A., Levine, J. D., & Julius, D. (1997) · Nature · doi:10.1038/39807 · Cited by 9,107
The Cloned Capsaicin Receptor Integrates Multiple Pain-Producing Stimuli
Tominaga, M., Caterina, M. J., Malmberg, A. B., Rosen, T. A., Gilbert, H., Skinner, K., Raumann, B. E., Basbaum, A. I., & Julius, D. (1998) · Neuron · doi:10.1016/s0896-6273(00)80564-4 · Cited by 3,081
A capsaicin-receptor homologue with a high threshold for noxious heat
Caterina, M. J., Rosen, T. A., Tominaga, M., Brake, A. J., & Julius, D. (1999) · Nature · doi:10.1038/18906 · Cited by 1,603
Hyperpolarization-Activated Cation Currents: From Molecules to Physiological Function
Robinson, R. B., & Siegelbaum, S. A. (2003) · Annual Review of Physiology · doi:10.1146/annurev.physiol.65.092101.142734 · Cited by 1,177
Cyclic Nucleotide-Gated Ion Channels
Kaupp, U. B., & Seifert, R. (2002) · Physiological Reviews · doi:10.1152/physrev.00008.2002 · Cited by 1,143
OTRPC4, a nonselective cation channel that confers sensitivity to extracellular osmolarity
Strotmann, R., Harteneck, C., Nunnenmacher, K., Schultz, G., & Plant, T. D. (2000) · Nature Cell Biology · doi:10.1038/35036318 · Cited by 1,023
Self-citationTRPV3 is a temperature-sensitive vanilloid receptor-like protein
Smith, G. D., Gunthorpe, M. J., Kelsell, R. E., Hayes, P. D., Reilly, P., Facer, P., Wright, J. E., Jerman, J. C., Walhin, J.-P., Ooi, L., et al. (2002) · Nature · doi:10.1038/nature00894 · Cited by 836
Subunit composition of mammalian transient receptor potential channels in living cells
Hofmann, T., Schaefer, M., Schultz, G., & Gudermann, T. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.102596199 · Cited by 743
Self-citationTRPC1 and TRPC5 Form a Novel Cation Channel in Mammalian Brain
Strübing, C., Krapivinsky, G., Krapivinsky, L., & Clapham, D. E. (2001) · Neuron · doi:10.1016/s0896-6273(01)00240-9 · Cited by 738
Altered urinary bladder function in mice lacking the vanilloid receptor TRPV1
Birder, L. A., Nakamura, Y., Kiss, S., Nealen, M. L., Barrick, S., Kanai, A. J., Wang, E., Ruiz, G., de Groat, W. C., Apodaca, G., et al. (2002) · Nature Neuroscience · doi:10.1038/nn902 · Cited by 703
Cited by
Trp ion channels and temperature sensation
Dhaka, A., Viswanath, V., & Patapoutian, A. (2006) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.29.051605.112958 · Cited by 782
Nilius, B., & Szallasi, A. (2014) · Pharmacological Reviews · doi:10.1124/pr.113.008268 · Cited by 506
The Ankyrin Repeats of TRPV1 Bind Multiple Ligands and Modulate Channel Sensitivity
Lishko, P. V., Procko, E., Jin, X., Phelps, C. B., & Gaudet, R. (2007) · Neuron · doi:10.1016/j.neuron.2007.05.027 · Cited by 447
TRPV4: molecular conductor of a diverse orchestra
White, J. P. M., Cibelli, M., Urban, L., Nilius, B., McGeown, J. G., & Nagy, I. (2016) · Physiological Reviews · doi:10.1152/physrev.00016.2015 · Cited by 405
Capsaicin (TRPV1 Agonist) Therapy for Pain Relief: Farewell or Revival?
Knotkova, H., Pappagallo, M., & Szallasi, A. (2008) · The Clinical Journal of Pain · doi:10.1097/ajp.0b013e318158ed9e · Cited by 279
Metadata
- Authors
- Hellwig, N., Albrecht, N., Harteneck, C., Schultz, G., & Schaefer, M.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 2005-03-01
- Volume
- 118
- Issue
- 5
- Pages
- 917-928
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W1971826341
- Cited by
- 303
- References
- 47
- FWCI
- 11.07
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Homo- and heteromeric assembly of TRPV channel subunits. https://app.cochat.ai/papers/10.1242/jcs.01675
- The paper
- Hellwig, N., Albrecht, N., Harteneck, C., Schultz, G., & Schaefer, M. (2005). Homo- and heteromeric assembly of TRPV channel subunits. Journal of Cell Science. https://doi.org/10.1242/jcs.01675
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
