The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Podoplanin binds ERM proteins to activate RhoA and promote epithelial-mesenchymal transition
Open accessAbstract
Podoplanin is a small membrane mucin expressed in tumors associated with malignant progression. It is enriched at cell-surface protrusions where it colocalizes with members of the ERM (ezrin, radixin, moesin) protein family. Here, we found that human podoplanin directly interacts with ezrin (and moesin) in vitro and in vivo through a cluster of basic amino acids within its cytoplasmic tail, mainly through a juxtamembrane dipeptide RK. Podoplanin induced an epithelial-mesenchymal transition in MDCK cells linked to the activation of RhoA and increased cell migration and invasiveness. Fluorescence time-lapse video observations in migrating cells indicate that podoplanin might be involved in ruffling activity as well as in retractive processes. By using mutant podoplanin constructs fused to green fluorescent protein we show that association of the cytoplasmic tail with ERM proteins is required for upregulation of RhoA activity and epithelial-mesenchymal transition. Furthermore, expression of either a dominant-negative truncated variant of ezrin or a dominant-negative mutant form of RhoA blocked podoplanin-induced RhoA activation and epithelial-mesenchymal transition. These results provide a mechanistic basis to understand the role of podoplanin in cell migration or invasiveness.
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Cites
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Cited by
Epithelial-Mesenchymal Transitions in Development and Disease
Thiery, J. P., Acloque, H., Huang, R. Y. J., & Nieto, M. A. (2009) · Cell · doi:10.1016/j.cell.2009.11.007 · Cited by 9,931
Cancer Invasion and the Microenvironment: Plasticity and Reciprocity
Friedl, P., & Alexander, S. (2011) · Cell · doi:10.1016/j.cell.2011.11.016 · Cited by 1,940
The Osteocyte: An Endocrine Cell … and More
Dallas, S. L., Prideaux, M., & Bonewald, L. F. (2013) · Endocrine Reviews · doi:10.1210/er.2012-1026 · Cited by 1,042
Organizing the cell cortex: the role of ERM proteins
Fehon, R. G., McClatchey, A. I., & Bretscher, A. (2010) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2866 · Cited by 1,044
Mechanisms of Motility in Metastasizing Cells
Yilmaz, M., & Christofori, G. (2010) · Molecular Cancer Research · doi:10.1158/1541-7786.mcr-10-0139 · Cited by 477
Metadata
- Authors
- Martín-Villar, E., Megías, D., Castel, S., Yurrita, M. M., Vilaró, S., & Quintanilla, M.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 2006-11-01
- Volume
- 119
- Issue
- 21
- Pages
- 4541-4553
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2084558110
- Cited by
- 379
- References
- 50
- FWCI
- 4.67
- Type
- journal article
- Language
- en
- Open access
- green
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- Martín-Villar, E., Megías, D., Castel, S., Yurrita, M. M., Vilaró, S., & Quintanilla, M. (2006). Podoplanin binds ERM proteins to activate RhoA and promote epithelial-mesenchymal transition. Journal of Cell Science. https://doi.org/10.1242/jcs.03218
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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