The DOI matches its Crossref record; 74 of the 74 references it cites were found in a registry or an index.
Mechanically induced osteogenic differentiation – the role of RhoA, ROCKII and cytoskeletal dynamics
Open accessAbstract
Many biochemical factors regulating progenitor cell differentiation have been examined in detail; however, the role of the local mechanical environment on stem cell fate has only recently been investigated. In this study, we examined whether oscillatory fluid flow, an exogenous mechanical signal within bone, regulates osteogenic, adipogenic or chondrogenic differentiation of C3H10T1/2 murine mesenchymal stem cells by measuring Runx2, PPARγ and SOX9 gene expression, respectively. Furthermore, we hypothesized that the small GTPase RhoA and isometric tension within the actin cytoskeleton are essential in flow-induced differentiation. We found that oscillatory fluid flow induces the upregulation of Runx2, Sox9 and PPARγ, indicating that it has the potential to regulate transcription factors involved in multiple unique lineage pathways. Furthermore, we demonstrate that the small GTPase RhoA and its effector protein ROCKII regulate fluid-flow-induced osteogenic differentiation. Additionally, activated RhoA and fluid flow have an additive effect on Runx2 expression. Finally, we show RhoA activation and actin tension are negative regulators of both adipogenic and chondrogenic differentiation. However, an intact, dynamic actin cytoskeleton under tension is necessary for flow-induced gene expression.
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Cites
Osf2/Cbfa1: A Transcriptional Activator of Osteoblast Differentiation
Ducy, P., Zhang, R., Geoffroy, V., Ridall, A. L., & Karsenty, G. (1997) · Cell · doi:10.1016/s0092-8674(00)80257-3 · Cited by 4,246
Komori, T., Yagi, H., Nomura, S., Yamaguchi, A., Sasaki, K., Deguchi, K., Shimizu, Y., Bronson, R. T., Gao, Y.-H., Inada, M., et al. (1997) · Cell · doi:10.1016/s0092-8674(00)80258-5 · Cited by 4,244
Cell Shape, Cytoskeletal Tension, and RhoA Regulate Stem Cell Lineage Commitment
McBeath, R., Pirone, D. M., Nelson, C. M., Bhadriraju, K., & Chen, C. S. (2004) · Developmental Cell · doi:10.1016/s1534-5807(04)00075-9 · Cited by 4,180
Otto, F., Thornell, A. P., Crompton, T., Denzel, A., Gilmour, K. C., Rosewell, I. R., Stamp, G. W. H., Beddington, R. S. P., Mundlos, S., Olsen, B. R., et al. (1997) · Cell · doi:10.1016/s0092-8674(00)80259-7 · Cited by 2,814
Cellular mechanotransduction: putting all the pieces together again
Ingber, D. E. (2006) · The FASEB Journal · doi:10.1096/fj.05-5424rev · Cited by 1,627
A model for the excitation of osteocytes by mechanical loading-induced bone fluid shear stresses
Weinbaum, S., Cowin, S. C., & Zeng, Y. (1994) · Journal of Biomechanics · doi:10.1016/0021-9290(94)90010-8 · Cited by 1,277
The Rho family GTPases RhoA, Racl , and CDC42Hsregulate transcriptional activation by SRF
Hill, C. S., Wynne, J., & Treisman, R. (1995) · Cell · doi:10.1016/s0092-8674(05)80020-0 · Cited by 1,269
Endochondral ossification: How cartilage is converted into bone in the developing skeleton
Mackie, E. J., Ahmed, Y. A., Tatarczuch, L., Chen, K.-S., & Mirams, M. (2008) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2007.06.009 · Cited by 1,022
Vinculin controls focal adhesion formation by direct interactions with talin and actin
Humphries, J. D., Wang, P., Streuli, C., Geiger, B., Humphries, M. J., & Ballestrem, C. (2007) · The Journal of Cell Biology · doi:10.1083/jcb.200703036 · Cited by 909
Cadherin-catenin complex: Protein interactions and their implications for cadherin function
Aberle, H., Schwartz, H., & Kemler, R. (1996) · Journal of Cellular Biochemistry · doi:10.1002/(sici)1097-4644(19960616)61:4<514::aid-jcb4>3.0.co;2-r · Cited by 806
Cited by
Geometric cues for directing the differentiation of mesenchymal stem cells
Kilian, K. A., Bugarija, B., Lahn, B. T., & Mrksich, M. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0903269107 · Cited by 1,841
Sart, S., Tsai, A.-C., Li, Y., & Ma, T. (2014) · Tissue Engineering Part B: Reviews · doi:10.1089/ten.teb.2013.0537 · Cited by 382
Metadata
- Authors
- Arnsdorf, E. J., Tummala, P., Kwon, R. Y., & Jacobs, C. R.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 2009-02-15
- Volume
- 122
- Issue
- 4
- Pages
- 546-553
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2130804510
- Cited by
- 396
- References
- 74
- FWCI
- 11.30
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Arnsdorf, E. J., Tummala, P., Kwon, R. Y., & Jacobs, C. R. (2009). Mechanically induced osteogenic differentiation – the role of RhoA, ROCKII and cytoskeletal dynamics. Journal of Cell Science. https://doi.org/10.1242/jcs.036293
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