RalA and the exocyst complex influence neuronal polarity through PAR-3 and aPKC
Abstract
Neuronal polarization requires localized cytoskeletal changes and polarized membrane traffic. Here, I report that the small GTPase RalA, previously shown to control neurite branching, also regulates neuronal polarity. RalA depletion, or ectopic expression of constitutively active RalA in cultured neurons inhibit axon formation. However, expression of a constitutively active RalA mutant that is unable to interact with the exocyst complex has no effect on neuronal polarization. Furthermore, depletion of the Sec6, Sec8 or Exo84 subunits of the exocyst complex also leads to unpolarized neurons. Early stages of neuronal polarization are accompanied by increasing levels of interaction of the exocyst complex with PAR-3 and atypical protein kinase C (aPKC), and by the RalA-dependent association of the exocyst complex with PAR-3. Thus, neuronal polarization involves a RalA-regulated association between mediators of vesicle trafficking (exocyst complex) and cell polarity (PAR-3).
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Ral, via the exocyst, promotes neurite branching and participates in establishing neuronal polarity.
“Ral, via the exocyst, promotes neurite branching and participates in establishing neuronal polarity”
From Ral mediates activity‐dependent growth of postsynaptic membranes via recruitment of the exocyst (2013) · cites this paper as Lalli, 2009
Neurite outgrowth depends on exocytosis to deliver membrane materials to specific surface regions, a process requiring RalA and exocyst.
“Neurite outgrowth depends on exocytosis to deliver membrane materials to specific surface regions, a process requiring RalA and exocyst (53–55).”
From Regulation of cargo exocytosis by a Reps1-Ralbp1-RalA module (2023) · cites this paper as [53]
Cites
The sequential activity of the GTPases Rap1B and Cdc42 determines neuronal polarity.
Schwamborn, J. C., & Püschel, A. W. (2004) · Nature Neuroscience · doi:10.1038/nn1295 · Cited by 385 · see citing works
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Lohia, M., Qin, Y., & Macara, I. G. (2012) · PLoS ONE · doi:10.1371/journal.pone.0051130 · Cited by 64 · see citing works
Ral mediates activity‐dependent growth of postsynaptic membranes via recruitment of the exocyst
Teodoro, R. O., Pekkurnaz, G., Nasser, A., Higashi‐Kovtun, M. E., Balakireva, M., McLachlan, I. G., Camonis, J., & Schwarz, T. L. (2013) · The EMBO Journal · doi:10.1038/emboj.2013.147 · Cited by 61 · see citing works
Regulation of cargo exocytosis by a Reps1-Ralbp1-RalA module
Wang, S., Chen, X., Crisman, L., Dou, X., Winborn, C. S., Wan, C., Puscher, H., Yin, Q., Kennedy, M. J., & Shen, J. (2023) · Science Advances · doi:10.1126/sciadv.ade2540 · Cited by 14 · see citing works
- Authors
- Lalli, G.
- Affiliations
- King's College London (GB)
- Corresponding author
- Lalli, Giovanna
- ORCID
- Lalli, Giovanna
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 2009-05-15
- Volume
- 122
- Issue
- 10
- Pages
- 1499-1506
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2089693284
- Cited by
- 84
- References
- 1
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 7 · 2020: 1 · 2019: 2 · 2018: 6 · 2017: 11 · 2016: 6 · 2015: 3 · 2014: 9 · 2013: 6 · 2012: 12
- FWCI
- 3.46
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Cell Science (publishedVersion)
- PubMed (publishedVersion)
- Research Portal (King's College London) (submittedVersion)
- HighWire Press Open Archive (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- RalA, exocyst complex, neuronal polarity, axon formation, Par3, atypical protein kinase C, small GTPases, vesicle trafficking, Sec6, Sec8, neurite branching
- MeSH terms
- Animals, Axons, Carrier Proteins, Cells, Cultured, Cerebral Cortex, Membrane Proteins, Mutation, Nerve Tissue Proteins, Neurons, Protein Kinase C, Time Factors, Signal Transduction, Cell Polarity, ral GTP-Binding Proteins, Transport Vesicles, Vesicular Transport Proteins, RNA Interference, RNA, Small Interfering, Rats
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