The life of an mRNA in space and time
Open accessAbstract
Nuclear transcribed genes produce mRNA transcripts destined to travel from the site of transcription to the cytoplasm for protein translation. Certain transcripts can be further localized to specific cytoplasmic regions. We examined the life cycle of a transcribed β-actin mRNA throughout gene expression and localization, in a cell system that allows the in vivo detection of the gene locus, the transcribed mRNAs and the cytoplasmic β-actin protein that integrates into the actin cytoskeleton. Quantification showed that RNA polymerase II elongation progressed at a rate of 3.3 kb/minute and that transactivator binding to the promoter was transient (40 seconds), and demonstrated the unique spatial structure of the coding and non-coding regions of the integrated gene within the transcription site. The rates of gene induction were measured during interphase and after mitosis, demonstrating that daughter cells were not synchronized in respect to transcription initiation of the studied gene. Comparison of the spatial and temporal kinetics of nucleoplasmic and cytoplasmic mRNA transport showed that the β-actin-localization response initiates from the existing cytoplasmic mRNA pool and not from the newly synthesized transcripts arising after gene induction. It was also demonstrated that mechanisms of random movement were predominant in mediating the efficient translocation of mRNA in the eukaryotic cell.
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RNA Pol II speed in live cells was estimated to range from 0.6 kb/min to 4.3 kb/min by FRAP experiments using MS2 fluorescent proteins.
“RNA Pol II speed in live cells was estimated to range from 0.6 kb/min to 4.3 kb/min by fluorescence recovery after photobleaching (FRAP) experiments using MS2 fluorescent proteins to label nascent MS2 repeat‐containing RNAs (Boireau et al, 2007; Darzacq et al, 2007; Ben‐Ari et al, 2010; Yunger et al, 2010; Brody et al, 2011; Maiuri et al, 2011; Muramoto et al, 2012; Corrigan et al, 2016).”
From RNA polymerase II speed: a key player in controlling and adapting transcriptome composition (2021) · cites this paper as Ben‐Ari et al, 2010
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The transcriptional cycle of HIV-1 in real-time and live cells
Boireau, S., Maiuri, P., Basyuk, E., de la Mata, M., Knezevich, A., Pradet-Balade, B., Bäcker, V., Kornblihtt, A., Marcello, A., & Bertrand, E. (2007) · The Journal of Cell Biology · doi:10.1083/jcb.200706018 · Cited by 191 · see citing works
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- Golding, I. and Cox, E. C. (2004). RNA dynamics in live Escherichia coli cells. Proc. Natl. Acad. Sci. USA 101, 11310-11315.
- Politz, J. C. and Pederson, T. (2000). Review: movement of mRNA from transcription site to nuclear pores. J. Struct. Biol. 129, 252-257.
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RNA polymerase II speed: a key player in controlling and adapting transcriptome composition
Muniz, L., Nicolas, E., & Trouche, D. (2021) · The EMBO Journal · doi:10.15252/embj.2020105740 · Cited by 203 · see citing works
“…recovery after photobleaching (FRAP) experiments using MS2 fluorescent proteins to label nascent MS2 repeat-containing RNAs (Boireau et al , 2007; Darzacq et al , 2007; Ben-Ari et al , 2010; Yunger et al , 2010; Brody et al , 2011; Maiuri et al , 2011; Muramoto et al , 2012; Corrigan et al , 2016).”
- Authors
- Ben-Ari, Y., Brody, Y., Kinor, N., Mor, A., Tsukamoto, T., Spector, D. L., Singer, R. H., & Shav-Tal, Y.
- Affiliations
- Bar-Ilan University (IL)
- Chiba University (JP)
- Cold Spring Harbor Laboratory (US)
- Albert Einstein College of Medicine (US)
- The Mina and Everard Goodman Faculty of Life Sciences and Institute of Nanotechnology, Bar-Ilan University, Ramat Gan 52900, Israel
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 2010-05-15
- Volume
- 123
- Issue
- 10
- Pages
- 1761-1774
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- PMC
- PMC2864715
- OpenAlex
- W2132918077
- Cited by
- 133
- References
- 1
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 9 · 2023: 4 · 2022: 9 · 2021: 6 · 2020: 10 · 2019: 16 · 2018: 2 · 2017: 10 · 2016: 8 · 2015: 8 · 2014: 9 · 2013: 9 · 2012: 9
- FWCI
- 4.13
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Cell Science (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Cold Spring Harbor Laboratory Institutional Repository (Cold Spring Harbor Laboratory) (acceptedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- U.S. Public Health Service (NIGMS 42694)
- National Institute of General Medical Sciences (R01GM042694, R01GM084364)
- National Institute of Biomedical Imaging and Bioengineering (EB2060, R01EB002060)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- mRNA localization, beta-actin, RNA polymerase II elongation, transcription initiation, gene induction, mRNA transport, actin cytoskeleton, mitosis, interphase, random diffusion, mRNA export, gene expression kinetics
- MeSH terms
- Actins, Cloning, Molecular, Cytoplasm, Humans, Lac Operon, Microscopy, Fluorescence, RNA, Messenger, Transcription, Genetic, Transcriptional Activation, Cell Line, Tumor
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