The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Analysis of p53 expression in human tumours: an antibody raised against human p53 expressed in Escherichia coli
Abstract
A cDNA encoding the complete normal human p53 protein was expressed in Escherichia coli using an expression system based on the bacteriophage T7 promoter. The cDNA was adapted so that the full-length protein was produced without fusion to any other sequence. Large amounts of the protein were isolated and the purified protein used to produce very high titre polyclonal antibodies to p53. These new antibodies permit the sensitive detection of p53 and p53 complexes in ELISA and immunoblotting assays. Most importantly, they also permit the detection of p53 in archival tumour material that has been conventionally fixed in formalin and embedded in paraffin wax. Using this reagent we have found that aberrant expression of p53 is a frequent feature of human breast cancer. We are able to recognise six different classes of p53 expression pattern that may be of help in the subclassification of breast tumours.
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Citations
Cites
p53 Mutations in Human Cancers
Hollstein, M., Sidransky, D., Vogelstein, B., & Harris, C. C. (1991) · Science · doi:10.1126/science.1905840 · Cited by 8,018
Cited by
p53 isoforms can regulate p53 transcriptional activity
Bourdon, J.-C., Fernandes, K., Murray-Zmijewski, F., Liu, G., Diot, A., Xirodimas, D. P., Saville, M. K., & Lane, D. P. (2005) · Genes & Development · doi:10.1101/gad.1339905 · Cited by 810
Self-citationp53 In tumour pathology: Can we trust immunohistochemistry?—revisited!
Hall, P. A., & Lane, D. P. (1994) · The Journal of Pathology · doi:10.1002/path.1711720103 · Cited by 561
Self-citationAre there low-penetrance TP53 alleles? Evidence from childhood adrenocortical tumors
Varley, J. M., McGown, G., Thorncroft, M., James, L. A., Margison, G. P., Forster, G., Evans, D. G. R., Harris, M., Kelsey, A. M., & Birch, J. M. (1999) · The American Journal of Human Genetics · doi:10.1086/302575 · Cited by 266
Different effects of p14ARF on the levels of ubiquitinated p53 and Mdm2 in vivo
Xirodimas, D., Saville, M. K., Edling, C., Lane, D. P., & Laín, S. (2001) · Oncogene · doi:10.1038/sj.onc.1204656 · Cited by 169
Self-citationGire, V., & Wynford-Thomas, D. (1998) · Molecular and Cellular Biology · doi:10.1128/mcb.18.3.1611 · Cited by 161
Metadata
- Authors
- Midgley, C. A., Fisher, C. J., Bártek, J., Vojtések, B., Lane, D., & Barnes, D. M.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 1992-01-01
- Volume
- 101
- Issue
- 1
- Pages
- 183-190
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W1947531295
- Cited by
- 330
- References
- 1
- FWCI
- 26.07
- Type
- journal article
- Language
- en
- Open access
- closed
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- Midgley, C. A., Fisher, C. J., Bártek, J., Vojtések, B., Lane, D., & Barnes, D. M. (1992). Analysis of p53 expression in human tumours: an antibody raised against human p53 expressed in Escherichia coli. Journal of Cell Science. https://doi.org/10.1242/jcs.101.1.183
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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