The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
A monoclonal antibody recognizing nuclear matrix-associated nuclear bodies
Abstract
We have isolated a monoclonal antibody, 5E10, that labels discrete spots in the interphase nucleus. By immunoblotting mAb SE10 recognized predominantly a 126 kDa polypeptide with an isoelectric point of 5.5. Indirect immunofluorescence showed that mAb 5E10 labeled spots in many cell lines and tissues from rat or human origin, but not in cells from mouse, chicken, African green monkey, or the lower eukaryotes Saccharomyces and Dictyostelium. In the human bladder carcinoma cell line T24 the number of nuclear spots was found to be 21 ± 10 (n=132). In many cells spots were found also in the cytoplasm. In a small fraction of T24 cells the mAb revealed thread-like structures in addition to spots. Throughout mitosis the antigen was found to be clustered in the cytoplasm, not associated with metaphase chromosomes. The spherical structures that contain the antigen were tightly bound to the nuclear matrix. Immunogold labeling with mAb 5E10 showed that the antigen is localized in 0.3 /mi diameter spherical, electron-dense structures, reminiscent of nuclear bodies. Double-labeling experiments showed that these spots do not colocalize with U1 snRNPs and centromeres. The spots did colocalize with nuclear speckles recognized by a primary biliary cirrhosis autoimmune serum, which is thought to recognize nuclear bodies. On the basis of these observations we conclude that mAb 5E10 recognizes discrete nuclear substructures, most likely nuclear bodies.
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Cited by
Ishov, A. M., Sotnikov, A. G., Negorev, D., Vladimirova, O. V., Neff, N., Kamitani, T., Yeh, E. T. H., Strauss, J. F., & Maul, G. G. (1999) · The Journal of Cell Biology · doi:10.1083/jcb.147.2.221 · Cited by 832
A novel macromolecular structure is a target of the promyelocyte-retinoic acid receptor oncoprotein
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Nuclear Shape, Mechanics, and Mechanotransduction
Dahl, K. N., Ribeiro, A. J. S., & Lammerding, J. (2008) · Circulation Research · doi:10.1161/circresaha.108.173989 · Cited by 708
Chromatin Motion Is Constrained by Association with Nuclear Compartments in Human Cells
Chubb, J. R., Boyle, S., Perry, P., & Bickmore, W. A. (2002) · Current Biology · doi:10.1016/s0960-9822(02)00695-4 · Cited by 580
Lallemand-Breitenbach, V., Zhu, J., Puvion, F., Koken, M., Honoré, N., Doubeikovsky, A., Duprez, E., Pandolfi, P. P., Puvion, E., Freemont, P., et al. (2001) · The Journal of Experimental Medicine · doi:10.1084/jem.193.12.1361 · Cited by 488
Metadata
- Authors
- Stuurman, N., Graaf, A. D., Floore, A., Josso, A., Humbel, B., Jong, L. D., & Driel, R. V.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 1992-04-01
- Volume
- 101
- Issue
- 4
- Pages
- 773-784
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2261355130
- Cited by
- 256
- References
- 39
- FWCI
- 2.80
- Type
- journal article
- Language
- en
- Open access
- closed
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- Stuurman, N., Graaf, A. D., Floore, A., Josso, A., Humbel, B., Jong, L. D., & Driel, R. V. (1992). A monoclonal antibody recognizing nuclear matrix-associated nuclear bodies. Journal of Cell Science. https://doi.org/10.1242/jcs.101.4.773
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