The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Development and characterisation of a rat brain capillary endothelial culture: towards an in vitro blood-brain barrier
Abstract
Primary culture of rat brain endothelial cells is described, based on the method of C. C. W. Hughes and P. L. Lantos. The cells have been characterised using morphological and immunocytochemical techniques, and systematic studies undertaken to determine the optimal culture medium and conditions required to grow the cells at high purity on a variety of substrata. The endothelial cells have a spindle-shaped morphology, and proliferate as plaques from small clusters of cells associated with capillary fragments in the starting material. Tight junction-like celkcell appositions are seen at the electron-microscopic level. The cells show characteristic staining for antigens recognized by antibodies against von Willebrand factor (Factor VTH-related antigen), angiotensin-converting enzyme (ACE), the transferrin receptor (Ox-26), actin and vimentin. They also show binding of the lectin from Ulex europaeus (UEA I). Potential contaminating cells include smooth muscle, fibroblasts, pericytes and meningeal cells. Contaminants can be kept to < ca. 5% by careful removal of large vessels and meninges during dissection, by brief treatment with Ca2+- and Mg2+-free saline, by growth in medium supplemented with plasma-derived serum treated for removal of platelet-derived growth factor (PDGF), and by occasional use of medium in which D-valine is substituted for L-valine. Cells attach well to collagen-coated plastic, less well to glass. Cells can be grown on transparent collagen filters (ICN, Cellagen and Costar, Transwell-Col), and on microcarrier beads (Pharmacia, Cytodex-3). The culture has proved to be a useful preparation for studies of cellular physiology, pharmacology and biochemistry of the brain endothelium, and represents a first step in producing an in vitro model of the rat blood-brain barrier.
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Cites
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A cell culture model of the blood-brain barrier.
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Glial Cell Diversification in the Rat Optic Nerve
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An Easier, Reproducible, and Mass‐Production Method to Study the Blood–Brain Barrier In Vitro
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Cited by
Helms, H. C., Abbott, N. J., Burek, M., Cecchelli, R., Couraud, P.-O., Deli, M. A., Förster, C., Galla, H. J., Romero, I. A., Shusta, E. V., et al. (2016) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1177/0271678x16630991 · Cited by 843
Self-citationMicrobiome–host systems interactions: protective effects of propionate upon the blood–brain barrier
Hoyles, L., Snelling, T., Umlai, U.-K., Nicholson, J. K., Carding, S. R., Glen, R. C., & McArthur, S. (2018) · Microbiome · doi:10.1186/s40168-018-0439-y · Cited by 591
Brown, R. C., Morris, A. P., & O'Neil, R. G. (2007) · Brain Research · doi:10.1016/j.brainres.2006.10.083 · Cited by 291
Nagasawa, K., Chiba, H., Fujita, H., Kojima, T., Saito, T., Endo, T., & Sawada, N. (2006) · Journal of Cellular Physiology · doi:10.1002/jcp.20647 · Cited by 255
MicroRNA‐155 negatively affects blood–brain barrier function during neuroinflammation
Lopez‐Ramirez, M. A., Wu, D., Pryce, G., Simpson, J. E., Reijerkerk, A., King‐Robson, J., Kay, O., de Vries, H. E., Hirst, M. C., Sharrack, B., et al. (2014) · The FASEB Journal · doi:10.1096/fj.13-248880 · Cited by 233
Metadata
- Authors
- Abbott, N. J., Hughes, C. C. W., Revest, P. A., & Greenwood, J.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 1992-09-01
- Volume
- 103
- Issue
- 1
- Pages
- 23-37
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W1907048502
- Cited by
- 318
- References
- 43
- FWCI
- 4.56
- Type
- journal article
- Language
- en
- Open access
- closed
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- Abbott, N. J., Hughes, C. C. W., Revest, P. A., & Greenwood, J. (1992). Development and characterisation of a rat brain capillary endothelial culture: towards an in vitro blood-brain barrier. Journal of Cell Science. https://doi.org/10.1242/jcs.103.1.23
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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