The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Active RNA polymerases are localized within discrete transcription ‘factories’ in human nuclei
Open accessAbstract
Nascent transcripts in permeabilized HeLa cells were elongated by ∼30-2,000 nucleotides in Br-UTP or biotin-14-CTP, before incorporation sites were immunolabelled either pre- or post-embedding, and visualized by light or electron microscopy. Analogues were concentrated in ∼2,100 (range 2,000-2,700) discrete sites attached to a nucleoskeleton and surrounded by chromatin. A typical site contained a cluster (diameter 71 nm) of at least 4, and probably about 20, engaged polymerases, plus associated transcripts that partially overlapped a zone of RNA polymerase II, ribonucleoproteins, and proteins rich in thiols and acidic groups. As each site probably contains many transcription units, these results suggest that active polymerases are confined to these sites, which we call transcription ‘factories’. Results are consistent with transcription occurring as templates slide past attached polymerases, as nascent RNA is extruded into the factories.
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Cites
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Self-citationMacromolecular Domains within the Cell Nucleus
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Cited by
Exploring the three-dimensional organization of genomes: Interpreting chromatin interaction data
Dekker, J., Marti-Renom, M. A., & Mirny, L. A. (2013) · Nature Reviews Genetics · doi:10.1038/nrg3454 · Cited by 1,068
Functional and Mechanistic Diversity of Distal Transcription Enhancers
Bulger, M., & Groudine, M. (2011) · Cell · doi:10.1016/j.cell.2011.01.024 · Cited by 803
Dynamic genome architecture in the nuclear space: regulation of gene expression in three dimensions
Lanctôt, C., Cheutin, T., Cremer, M., Cavalli, G., & Cremer, T. (2007) · Nature Reviews Genetics · doi:10.1038/nrg2041 · Cited by 781
Nuclear organization of the genome and the potential for gene regulation
Fraser, P., & Bickmore, W. (2007) · Nature · doi:10.1038/nature05916 · Cited by 734
Long-range chromatin regulatory interactions in vivo
Carter, D., Chakalova, L., Osborne, C. S., Dai, Y.-F., & Fraser, P. (2002) · Nature Genetics · doi:10.1038/ng1051 · Cited by 663
Metadata
- Authors
- Iborra, F. J., Pombo, A., Jackson, D. A., & Cook, P. R.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 1996-06-01
- Volume
- 109
- Issue
- 6
- Pages
- 1427-1436
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2146404878
- Cited by
- 458
- References
- 32
- FWCI
- 4.82
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Iborra, F. J., Pombo, A., Jackson, D. A., & Cook, P. R. (1996). Active RNA polymerases are localized within discrete transcription ‘factories’ in human nuclei. Journal of Cell Science. https://doi.org/10.1242/jcs.109.6.1427
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
