The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Occludin confers adhesiveness when expressed in fibroblasts
Abstract
Occludin is an integral membrane protein specifically associated with tight junctions. Previous studies suggest it is likely to function in forming the intercellular seal. In the present study, we expressed occludin under an inducible promotor in occludin-null fibroblasts to determine whether this protein confers intercellular adhesion. When human occludin is stably expressed in NRK and Rat-1 fibroblasts, which lack endogenous occludin and tight junctions but do have well developed ZO-1-containing adherens-like junctions, occludin colocalizes with ZO-1 to points of cellcell contact. In contrast, L-cell fibroblasts which lack cadherin-based adherens junctions, target neither ZO-1 nor occludin to sites of cell contact. Occludin-induced adhesion was next quantified using a suspended cell assay. In NRK and Rat-1 cells, occludin expression induces adhesion in the absence of calcium, thus independent of cadherin-cadherin contacts. In contrast, L-cells are nonadhesive in this assay and show no increase in adhesion after induction of occludin expression. Binding of an antibody to the first of the putative extracellular loops of occludin confirmed that this sequence was exposed on the cell surface, and synthetic peptides containing the amino acid sequence of this loop inhibit adhesion induced by occludin expression. These results suggest that the extracellular surface of occludin is directly involved in cell-cell adhesion and the ability to confer adhesiveness correlates with the ability to colocalize with its cytoplasmic binding protein, ZO-1.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Towbin, H., Staehelin, T., & Gordon, J. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.9.4350 · Cited by 53,385
Occludin: a novel integral membrane protein localizing at tight junctions.
Furuse, M., Hirase, T., Itoh, M., Nagafuchi, A., Yonemura, S., Tsukita, S., & Tsukita, S. (1993) · The Journal of cell biology · doi:10.1083/jcb.123.6.1777 · Cited by 2,580
Stevenson, B. R., Siliciano, J. D., Mooseker, M. S., & Goodenough, D. A. (1986) · The Journal of cell biology · doi:10.1083/jcb.103.3.755 · Cited by 1,638
Roy, N., Mahadevan, M. S., McLean, M., Shutter, G., Yaraghi, Z., Farahani, R., Baird, S., Besner-Johnston, A., Lefebvre, C., Kang, X., et al. (1995) · Cell · doi:10.1016/0092-8674(95)90461-1 · Cited by 981
Furuse, M., Itoh, M., Hirase, T., Nagafuchi, A., Yonemura, S., Tsukita, S., & Tsukita, S. (1994) · The Journal of cell biology · doi:10.1083/jcb.127.6.1617 · Cited by 962
Chen, C. A., & Okayama, H. (1988) · PubMed · Cited by 894
Balda, M. S., Whitney, J. A., Flores, C., González, S., Cereijido, M., & Matter, K. (1996) · The Journal of cell biology · doi:10.1083/jcb.134.4.1031 · Cited by 875
Tight junctions and the molecular basis for regulation of paracellular permeability
Anderson, J. M., & Van Itallie, C. M. (1995) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.1995.269.4.g467 · Cited by 843
Self-citationOccludin is a functional component of the tight junction
McCarthy, K. M., Skare, I. B., Stankewich, M. C., Furuse, M., Tsukita, S., Rogers, R. A., Lynch, R. D., & Schneeberger, E. E. (1996) · Journal of Cell Science · doi:10.1242/jcs.109.9.2287 · Cited by 645
Cited by
Intestinal barrier function: molecular regulation and disease pathogenesis
Groschwitz, K. R., & Hogan, S. P. (2009) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2009.05.038 · Cited by 1,775
Furuse, M., Sasaki, H., Fujimoto, K., & Tsukita, S. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.143.2.391 · Cited by 965
Der p 1 facilitates transepithelial allergen delivery by disruption of tight junctions
Wan, H., Winton, H. L., Soeller, C., Tovey, E. R., Gruenert, D. C., Thompson, P. J., Stewart, G. A., Taylor, G. W., Garrod, D. R., Cannell, M. B., et al. (1999) · Journal of Clinical Investigation · doi:10.1172/jci5844 · Cited by 775
IL-1β Causes an Increase in Intestinal Epithelial Tight Junction Permeability
Al-Sadi, R. M., & Ma, T. Y. (2007) · The Journal of Immunology · doi:10.4049/jimmunol.178.7.4641 · Cited by 601
Junctional Adhesion Molecule Interacts with the PDZ Domain-containing Proteins AF-6 and ZO-1
Ebnet, K., Schulz, C. U., Meyer zu Brickwedde, M.-K., Pendl, G. G., & Vestweber, D. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m002363200 · Cited by 438
Metadata
- Authors
- Itallie, C. M. V., & Anderson, J. M.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 1997-05-01
- Volume
- 110
- Issue
- 9
- Pages
- 1113-1121
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W1936651842
- Cited by
- 271
- References
- 34
- FWCI
- 9.65
- Type
- journal article
- Language
- en
- Open access
- closed
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