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Cited by 965

The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.

A Single Gene Product, Claudin-1 or -2, Reconstitutes Tight Junction Strands and Recruits Occludin in Fibroblasts

Open access
Furuse, Mikio, Sasaki, Hiroyuki, Fujimoto, Kazushi, Tsukita, Shoichiro·1998·The Journal of Cell Biology
DOI 10.1083/jcb.143.2.391
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Abstract

Three integral membrane proteins, clau- din-1, -2, and occludin, are known to be components of tight junction (TJ) strands. To examine their ability to form TJ strands, their cDNAs were introduced into mouse L fibroblasts lacking TJs. Immunofluorescence microscopy revealed that both FLAG-tagged claudin-1 and -2 were highly concentrated at cell contact sites as planes through a homophilic interaction. In freeze-fracture replicas of these contact sites, well-developed networks of strands were identified that were similar to TJ strand networks in situ and were specifically labeled with anti-FLAG mAb. In glutaraldehyde-fixed samples, claudin-1–induced strands were largely associated with the protoplasmic (P) face as mostly continuous structures, whereas claudin-2–induced strands were discontinuous at the P face with complementary grooves at the extracellular (E) face which were occupied by chains of particles. Although occludin was also concentrated at cell contact sites as dots through its homophilic interaction, freeze-fracture replicas identified only a small number of short strands that were labeled with anti-occludin mAb. However, when occludin was cotransfected with claudin-1, it was concentrated at cell contact sites as planes to be incorporated into well- developed claudin-1–based strands. These findings suggested that claudin-1 and -2 are mainly responsible for TJ strand formation, and that occludin is an accessory protein in some function of TJ strands.

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Metadata

Authors
Furuse, M., Sasaki, H., Fujimoto, K., & Tsukita, S.
Venue
The Journal of Cell Biology
Publisher
Rockefeller University Press
Published
1998-10-19
Volume
143
Issue
2
Pages
391-401
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.143.2.391
OpenAlex
W2106500404
Cited by
965
References
52
FWCI
23.99
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: A Single Gene Product, Claudin-1 or -2, Reconstitutes Tight Junction Strands and Recruits Occludin in Fibroblasts. https://app.cochat.ai/papers/10.1083/jcb.143.2.391
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Furuse, M., Sasaki, H., Fujimoto, K., & Tsukita, S. (1998). A Single Gene Product, Claudin-1 or -2, Reconstitutes Tight Junction Strands and Recruits Occludin in Fibroblasts. The Journal of Cell Biology. https://doi.org/10.1083/jcb.143.2.391

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.