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Distinct temporal hierarchies in membrane and cytoskeleton dynamics precede the morphological polarization of developing neurons

Gärtner, Annette, Fornasiero, Eugenio F., Valtorta, Flavia, Dotti, Carlos G.·2014·Journal of Cell Science
DOI 10.1242/jcs.149815
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Abstract

Final morphological polarization of neurons, with the development of a distinct axon and of several dendrites, is preceded by phases of non-polarized architecture. The earliest of these phases is that of the round neuron arising from the last mitosis. A second non polarized stage corresponds to the bipolar neuron, with two morphologically identical neurites. Both phases have their distinctive relevance in the establishment of neuronal polarity. During the round cell stage a decision is made as to where from the cell periphery a first neurite will form, thus creating the first sign of asymmetry. At the bipolar stage a decision is made as to which of the two neurites becomes the axon in neurons polarizing in vitro and the leading edge in neurons in situ. In this study we analysed cytoskeletal and membrane dynamics in cells at these two “pre-polarity” stages. By mean of time lapse imaging in dissociated hippocampal neurons and ex vivo cortical slices we show that both stages are characterized by polarized intracellular arrangements, however with distinct temporal hierarchies: polarized actin dynamics marks the site of first polarization in round cells, whereas polarized membrane dynamics precedes asymmetric growth in the bipolar stage.

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  • The process of neuronal polarization begins very early in the maturing neuron's life, which is when miR-338-3p expression is increasing.

    “The process of neuronal polarization begins very early in the maturing neuron’s life, which is when miR-338-3p expression is increasing [27].”

    From MiR-338-3p regulates neuronal maturation and suppresses glioblastoma proliferation. (2017) · cites this paper as [27]

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Cited by

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Authors
Gärtner, A., Fornasiero, E. F., Valtorta, F., & Dotti, C. G.
Venue
Journal of Cell Science
Publisher
The Company of Biologists
Published
2014-01-01
ISSN
  • 1477-9137
  • 0021-9533
DOI
10.1242/jcs.149815
Cited by
21
References
1
Language
en

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