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Septins are involved at the early stages of macroautophagy in S. cerevisiae

Barve, Gaurav, Sridhar, Shreyas, Aher, Amol, Sahani, Mayurbhai H., Chinchwadkar, Sarika, Singh, Sunaina, K. N., Lakshmeesha, McMurray, Michael A., Manjithaya, Ravi·2018·Journal of Cell Science
DOI 10.1242/jcs.209098PMC PMC5868950

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Abstract

Autophagy is a conserved cellular degradation pathway wherein double-membrane vesicles called autophagosomes capture long-lived proteins, and damaged or superfluous organelles, and deliver them to the lysosome for degradation. Septins are conserved GTP-binding proteins involved in many cellular processes, including phagocytosis and the autophagy of intracellular bacteria, but no role in general autophagy was known. In budding yeast, septins polymerize into ring-shaped arrays of filaments required for cytokinesis. In an unbiased genetic screen and in subsequent targeted analysis, we found autophagy defects in septin mutants. Upon autophagy induction, pre-assembled septin complexes relocalized to the pre-autophagosomal structure (PAS) where they formed non-canonical septin rings at PAS. Septins also colocalized with autophagosomes, where they physically interacted with the autophagy proteins Atg8 and Atg9. When autophagosome degradation was blocked in septin-mutant cells, fewer autophagic structures accumulated, and an autophagy mutant defective in early stages of autophagosome biogenesis (atg1Δ), displayed decreased septin localization to the PAS. Our findings support a role for septins in the early stages of budding yeast autophagy, during autophagosome formation. This article has an associated First Person interview with the first author of the paper.

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Metadata

Authors
Barve, G., Sridhar, S., Aher, A., Sahani, M. H., Chinchwadkar, S., Singh, S., K. N., L., McMurray, M. A., & Manjithaya, R.
Affiliations
  • Jawaharlal Nehru Centre for Advanced Scientific Research 1 Molecular Biology and Genetics Unit , , Jakkur, Bangalore 560064 , India
  • University of Colorado, Anschutz Medical Campus 2 , Department of Cell and Developmental Biology , Aurora, CO 80045, USA
ORCID
Venue
Journal of Cell Science
Publisher
The Company of Biologists
Published
2018-02-15
Volume
131
Issue
4
ISSN
  • 1477-9137
  • 0021-9533
DOI
10.1242/jcs.209098
PMC
PMC5868950
Cited by
32
References
3
Language
en
Licence
creativecommons.org/licenses/by/3.0 (vor, 939 d)
Funders
  • Wellcome Trust
  • DBT India Alliance (509159-Z-09-Z)
  • Jawaharlal Nehru Centre for Advanced Scientific Research
  • Council of Scientific and Industrial Research

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