The septin cytoskeleton facilitates membrane retraction during motility and blebbing
Open accessAbstract
Increasing evidence supports a critical role for the septin cytoskeleton at the plasma membrane during physiological processes including motility, formation of dendritic spines or cilia, and phagocytosis. We sought to determine how septins regulate the plasma membrane, focusing on this cytoskeletal element's role during effective amoeboid motility. Surprisingly, septins play a reactive rather than proactive role, as demonstrated during the response to increasing hydrostatic pressure and subsequent regulatory volume decrease. In these settings, septins were required for rapid cortical contraction, and SEPT6-GFP was recruited into filaments and circular patches during global cortical contraction and also specifically during actin filament depletion. Recruitment of septins was also evident during excessive blebbing initiated by blocking membrane trafficking with a dynamin inhibitor, providing further evidence that septins are recruited to facilitate retraction of membranes during dynamic shape change. This function of septins in assembling on an unstable cortex and retracting aberrantly protruding membranes explains the excessive blebbing and protrusion observed in septin-deficient T cells.
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Functional perturbation of proteins that direct the formation and/or regulation of the cytokinetic furrow results in similar plasma membrane blebbing during mitosis.
“Previous studies have demonstrated that functional perturbation of proteins that direct the formation and/or regulation of the cytokinetic furrow results in similar plasma membrane blebbing during mitosis [17, 28].”
From Tumor Treating Fields Perturb the Localization of Septins and Cause Aberrant Mitotic Exit (2015) · cites this paper as [28]
Knockdown of Septin 2, 6 or 7 results in similar violent membrane blebbing and abnormal mitotic exit.
“Indeed, knockdown of Septin 2, 6 or 7 has been shown to result in similar violent membrane blebbing and abnormal mitotic exit [28, 29]”
From Tumor Treating Fields Perturb the Localization of Septins and Cause Aberrant Mitotic Exit (2015) · cites this paper as [28]
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Tumor Treating Fields Perturb the Localization of Septins and Cause Aberrant Mitotic Exit
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- Authors
- Gilden, J. K., Peck, S., M. Chen, Y.-C., & Krummel, M. F.
- Affiliations
- University of California, San Francisco (US)
- Department of Pathology 1 and 2
- Corresponding author
- Krummel, Matthew F.
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2012-01-09
- Volume
- 196
- Issue
- 1
- Pages
- 103-114
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC3255977
- OpenAlex
- W2099127112
- Cited by
- 113
- References
- 49
- Citations by year
- 2026: 8 · 2025: 1 · 2024: 5 · 2023: 11 · 2022: 14 · 2021: 9 · 2020: 7 · 2019: 5 · 2018: 10 · 2017: 5 · 2016: 11 · 2015: 11 · 2014: 12 · 2013: 3 · 2012: 1
- FWCI
- 4.09
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion, cc-by-nc-sa)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- eScholarship (California Digital Library) (submittedVersion, cc-by-nc-sa)
- Funders
- National Science Foundation
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases (R01AI52116, R01AI052116)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- septins, amoeboid movement, blebbing, cortical contraction, regulatory volume decrease, hydrostatic pressure, membrane trafficking, plasma membrane
- MeSH terms
- Actomyosin, Animals, Biological Transport, Cell Line, Cell Membrane, Cell Movement, Cytoskeleton, Recombinant Fusion Proteins, Green Fluorescent Proteins, Mice, Septins
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