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TBC1D5 controls the GTPase cycle of Rab7b

Borg Distefano, Marita, Hofstad Haugen, Linda, Wang, Yan, Perdreau-Dahl, Harmonie, Kjos, Ingrid, Jia, Da, Morth, Jens Preben, Neefjes, Jacques, Bakke, Oddmund, Progida, Cinzia·2018·Journal of Cell Science
DOI 10.1242/jcs.216630
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Abstract

Rab GTPases are key regulators of intracellular trafficking, and cycle between a GTP-bound active state and a GDP-bound inactive state. This cycle is regulated by guanine-nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). Several efforts have been made in connecting the correct GEFs and GAPs to their specific Rab. Here, we aimed to identify GAPs for Rab7b, the small GTPase involved in transport from late endosomes to the trans-Golgi. An siRNA screen targeting proteins containing TBC domains critical for Rab GAPs was performed and coupled to a phenotypic read-out that visualized the distribution of Rab7b. Silencing of TBC1D5 provided the strongest phenotype and this protein was subsequently validated in various in vitro and cell-based assays. TBC1D5 localizes to Rab7b-positive vesicles, interacts with Rab7b and has GAP activity towards Rab7b in vitro, which is further increased by retromer proteins. Similarly to the constitutively active mutant of Rab7b, inactivation of TBC1D5 also reduces the number of CI-MPR- and sortilin-positive vesicles. Together, the results show that TBC1D5 is a GAP for Rab7b in the control of endosomal transport to the trans-Golgi. This article has an associated First Person interview with the first author of the paper.

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  • The Retromer sub-domain proximity proteome included the RAB7 GAP TBC1D5, the RAB21/RAB32/RAB38 GEF VARP, and the RAB10 GEFs DENND4A and DENND4C.

    “These included known Retromer accessory proteins, the RAB7 GAP TBC1D546,77–81, the RAB21/RAB32/RAB38 GEF VARP (a.k.a. ANKRD27)47,48,82, and the RAB10 GEFs DENND4A and DENND4C83,84.”

    From Mapping of endosomal proximity proteomes reveals Retromer as a hub for RAB GTPase regulation (2025) · cites this paper as [81]

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Metadata

Authors
Borg Distefano, M., Hofstad Haugen, L., Wang, Y., Perdreau-Dahl, H., Kjos, I., Jia, D., Morth, J. P., Neefjes, J., Bakke, O., & Progida, C.
Affiliations
  • Centre for Immune Regulation, University of Oslo 1 Department of Biosciences , , 0316 Oslo , Norway
  • Key Laboratory of Birth Defects and Related Diseases of Women and Children 2 , Department of Paediatrics , , Chengdu 610041 , China
  • Norwegian Center of Molecular Medicine, Nordic EMBL Partnership, University of Oslo 3 , 0318 Oslo , Norway
  • Leiden University Medical Center LUMC 5 Department of Cell and Chemical Biology , , 2300 RC Leiden , The Netherlands
ORCID
Venue
Journal of Cell Science
Publisher
The Company of Biologists
Published
2018-09-01
Volume
131
Issue
17
ISSN
  • 1477-9137
  • 0021-9533
DOI
10.1242/jcs.216630
Cited by
45
References
1
Language
en
Licence
www.biologists.com/user-licence-1-1/ (vor, 181 d)
Funders
  • Norwegian Cancer Society (5760850, 4604944)
  • Research Council of Norway (239903, 230779)

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