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Freeze-fracture study of phagocytosis in Dictyostelium discoideum

Favard-Séréno, Colette, Ludosky, Marie-Aline, Ryter, Antoinette·1981·Journal of Cell Science
DOI 10.1242/jcs.51.1.63
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Abstract

The plasma membrane and its derivative, the phagosome membrane, were studied during and after ingestion of yeast or latex beads in Dictyostelium discoideum. Freeze-fracture electron microscopy, which provides information on the internal architecture of the membranes, and observation of thin sections of cells treated by cytochemical methods were used in parallel. For visualization of membrane sterols in the replicas, the cells were fixed in the presence of digitonin or the antibiotic filipin. No lateral phase separation occurred during yeast engulfment: the intramembranous particles (IMPs), phospholipids and sterols remained distributed at random in the forming phagosome membrane. In contrast architectural modifications of the membrane were observed upon phagosome internalization. Compared to the plasma membrane, the phagosome membrane displayed 2–3 times more IMPs, a shift in the IMP size distribution and a higher sterol content. These changes were completed soon after phagosome closure; they were not related either to the nature of the ingested particles (yeast, latex beads) or to the pH in the membrane environment. The membrane changes took place when the phagosomes began to fuse with pre-existing digestive or autophagic vacuoles and lysosomes. Some of the experimental evidence suggests that the restructuring of the membrane may be related to the presence of hydrolases.

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Research works printed but not linked: 44

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Metadata

Authors
Favard-Séréno, C., Ludosky, M.-A., & Ryter, A.
Affiliations
Centre National de la Recherche Scientifique (FR)
Venue
Journal of Cell Science
Publisher
The Company of Biologists
Published
1981-10-01
Volume
51
Issue
1
Pages
63-84
ISSN
  • 0021-9533 (ISSN-L)
  • 1477-9137
DOI
10.1242/jcs.51.1.63
OpenAlex
W2416124942
Cited by
29
References
0
Citations by year
2020: 1
FWCI
1.53
Type
journal article
Language
en
Open access
closed
Licence
www.biologists.com/user-licence-1-1/ (vor)
Locations
  • Journal of Cell Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Slime Mold and Myxomycetes Research
Keywords
phagocytosis, Dictyostelium discoideum, plasma membrane, membrane sterols, intramembrane particles, membrane lipid distribution, phagosome-lysosome fusion, autophagosomes, hydrolytic enzymes, latex beads
MeSH terms
Cell Membrane, Chloroquine, Concanavalin A, Dictyostelium, Freeze Fracturing, Hydrogen-Ion Concentration, Microscopy, Electron, Microspheres, Phagocytosis, Saccharomyces cerevisiae, Sterols

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CoChat. (2026). Citation check: Freeze-fracture study of phagocytosis in Dictyostelium discoideum. https://app.cochat.ai/papers/10.1242/jcs.51.1.63
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Favard-Séréno, C., Ludosky, M.-A., & Ryter, A. (1981). Freeze-fracture study of phagocytosis in Dictyostelium discoideum. Journal of Cell Science. https://doi.org/10.1242/jcs.51.1.63