The DOI matches its Crossref record.
N-linked oligosaccharides are not involved in the function of a cell-cell binding glycoprotein E-cadherin.
Open accessAbstract
E-cadherin is a Ca2+-dependent cell-cell adhesion molecule identified as a glycoprotein with a molecular weight (MW) of 124,000. To study the role of the sugar moieties of this adhesion molecule, we tested the effect of tunicamycin on aggregation mediated by E-cadherin of teratocarcinoma cells. Immunoblot analysis using a monoclonal antibody to E-cadherin showed that in cells treated with tunicamycin this adhesion molecule is converted into two forms with MW of 118,000 and 131,000. The smaller one was exposed on the cell surface and showed a trypsin sensitivity characteristic to E-cadherin, suggesting that this is the peptide moiety of E-cadherin whose glycosylation with N-linked oligosaccharides was blocked by tunicamycin. The larger one was not removed by trypsin treatment of cells, suggesting an intracellular location. These tunicamycin-treated cells aggregated in a Ca2+-dependent manner, and the aggregation was inhibited by a monoclonal antibody to E-cadherin. These results suggested that N-linked oligosaccharides are not involved in the functional sites of this adhesion molecule.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Adhesion of epidermal Langerhans cells to keratinocytes mediated by E-cadherin
Tang, A., Amagai, M., Granger, L. G., Stanley, J. R., & Uddy, M. C. (1993) · Nature · doi:10.1038/361082a0 · Cited by 503
Induction and monitoring of definitive and visceral endoderm differentiation of mouse ES cells
Yasunaga, M., Tada, S., Torikai-Nishikawa, S., Nakano, Y., Okada, M., Jakt, L. M., Nishikawa, S., Chiba, T., Era, T., & Nishikawa, S.-I. (2005) · Nature Biotechnology · doi:10.1038/nbt1167 · Cited by 471
Tada, S., Era, T., Furusawa, C., Sakurai, H., Nishikawa, S., Kinoshita, M., Nakao, K., Chiba, T., & Nishikawa, S.-I. (2005) · Development · doi:10.1242/dev.02005 · Cited by 455
Watabe, M., Nagafuchi, A., Tsukita, S., & Takeichi, M. (1994) · The Journal of cell biology · doi:10.1083/jcb.127.1.247 · Cited by 399
Self-citationWatabe-Uchida, M., Uchida, N., Imamura, Y., Nagafuchi, A., Fujimoto, K., Uemura, T., Vermeulen, S., van Roy, F., Adamson, E. D., & Takeichi, M. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.142.3.847 · Cited by 348
Self-citation
Metadata
- Authors
- Shirayoshi, Y., Nose, A., Iwasaki, K., & Takeichi, M.
- Venue
- Cell Structure and Function
- Publisher
- Japan Society for Cell Biology
- Published
- 1986
- Volume
- 11
- Issue
- 3
- Pages
- 245-252
- ISSN
- 0386-7196 (ISSN-L)
- 1347-3700
- OpenAlex
- W1973930300
- Cited by
- 171
- References
- 0
- FWCI
- 1.24
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: N-linked oligosaccharides are not involved in the function of a cell-cell binding glycoprotein E-cadherin. https://app.cochat.ai/papers/10.1247/csf.11.245
- The paper
- Shirayoshi, Y., Nose, A., Iwasaki, K., & Takeichi, M. (1986). N-linked oligosaccharides are not involved in the function of a cell-cell binding glycoprotein E-cadherin. Cell Structure and Function. https://doi.org/10.1247/csf.11.245
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
