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A Combined Assay of Cell Viability and in Vitro Cytotoxicity with a Highly Water-Soluble Tetrazolium Salt, Neutral Red and Crystal Violet.
Open accessAbstract
Cell viability and in vitro cytotoxicity assay methods were developed using a combination of dyes, 4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate (WST-1), neutral red (NR) and crystal violet (CV), with HeLa cells as a bioindicatior. As WST-1 produces a highly water soluble and non-cytotoxic formazan dye, ti allows each assay to be carried out in one culture dish. The combined cell viability assay using WST-1, NR and CV gave an absorbance that correlated linearly with the number of cells over the range 1000 to 50,000 cells/well. The combined assay was applied to the evaluation of IC50 values for sodium dodecyl sulfate as a model toxicant, which yielded similar values to those obtained with each assay independently.
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Cited by
A water-soluble tetrazolium salt useful for colorimetric cell viability assay
Tominaga, H., Ishiyama, M., Ohseto, F., Sasamoto, K., Hamamoto, T., Suzuki, K., & Watanabe, M. (1999) · Analytical Communications · doi:10.1039/a809656b · Cited by 716
Self-citationIshiyama, M. (1997) · Talanta · doi:10.1016/s0039-9140(97)00017-9 · Cited by 687
Self-citationAu–Fe3O4 Dumbbell Nanoparticles as Dual‐Functional Probes
Xu, C., Xie, J., Ho, D., Wang, C., Kohler, N., Walsh, E. G., Morgan, J. R., Chin, Y. E., & Sun, S. (2008) · Angewandte Chemie International Edition · doi:10.1002/anie.200704392 · Cited by 526
In Vitro Cytotoxicity and Cell Viability Assays: Principles, Advantages, and Disadvantages
Aslantürk, Ö. S. (2018) · Genotoxicity - A Predictable Risk to Our Actual World · doi:10.5772/intechopen.71923 · Cited by 408
Nanotechnology, nanotoxicology, and neuroscience
Suh, W. H., Suslick, K. S., Stucky, G. D., & Suh, Y.-H. (2009) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2008.09.009 · Cited by 406
Metadata
- Authors
- ISHIYAMA, M., TOMINAGA, H., SHIGA, M., SASAMOTO, K., OHKURA, Y., & UENO, K.
- Venue
- Biological and Pharmaceutical Bulletin
- Publisher
- Pharmaceutical Society of Japan
- Published
- 1996
- Volume
- 19
- Issue
- 11
- Pages
- 1518-1520
- ISSN
- 0918-6158 (ISSN-L)
- 1347-5215
- OpenAlex
- W2000780867
- Cited by
- 737
- References
- 0
- FWCI
- 14.43
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: A Combined Assay of Cell Viability and in Vitro Cytotoxicity with a Highly Water-Soluble Tetrazolium Salt, Neutral Red and Crystal Violet. https://app.cochat.ai/papers/10.1248/bpb.19.1518
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- ISHIYAMA, M., TOMINAGA, H., SHIGA, M., SASAMOTO, K., OHKURA, Y., & UENO, K. (1996). A Combined Assay of Cell Viability and in Vitro Cytotoxicity with a Highly Water-Soluble Tetrazolium Salt, Neutral Red and Crystal Violet. Biological and Pharmaceutical Bulletin. https://doi.org/10.1248/bpb.19.1518
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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