Effects of Poly(ethylene glycol) (PEG) Concentration on the Permeability of PEG-Grafted Liposomes
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Poly(ethylene glycol)-grafted liposomes (PEG-liposomes) were prepared from dipalmitoylphosphatidylcholine (DPPC) with various amounts of distearoyl-N-monomethoxy poly(ethylene glycol)-succinyl-phosphatidylethanolamines (DSPE-PEG) with PEG molecular weights of 1000, 2000, 3000 and 5000. The effects of DSPE-PEG concentration on the permeability of PEG-liposomes were investigated using carboxyfluorescein (CF). In the gel state, the CF leakage from PEG-liposomes was decreased with increasing mole fractions of DSPE-PEG for all PEG molecular weights. In the liquid-crystalline state, the CF leakage from PEG-liposomes containing DSPE-PEG1000 gradually increased with increasing mole fractions of DSPE-PEG, while that of PEG-liposomes whose molecular weight in PEG units was above 2000 rapidly decreased by the addition of DSPE-PEG. Furthermore, no effect of PEG molecular weight on CF leakage was observed. The relationship between the fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) (or 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH)) and the mole fraction of DSPE-PEG for PEG-liposomes was also investigated. No significant changes in fluorescence polarization of DPH for liposomal bilayer membranes was observed in the gel and liquid-crystalline states due to the addition of DSPE-PEG, while that of TMA-DPH was decreased compared with that of liposomes without DSPE-PEG in both states.
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The main role of PEGylated lipid in LNPs is to reduce aggregation and non-specific uptake by immune cells.
“The main role of PEGylated lipid in LNPs is to reduce aggregation and non-specific uptake by immune cells.304–306”
From Advances in COVID-19 mRNA vaccine development (2022) · cites this paper as [304]
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Advances in COVID-19 mRNA vaccine development
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- Authors
- Hashizaki, K., Taguchi, H., Itoh, C., Sakai, H., Abe, M., Saito, Y., & Ogawa, N.
- Affiliations
- Nihon University (JP)
- NOF Corporation (Japan) (JP)
- Tokyo University of Science (JP)
- Venue
- Chemical and Pharmaceutical Bulletin
- Publisher
- Pharmaceutical Society of Japan
- Published
- 2005
- Volume
- 53
- Issue
- 1
- Pages
- 27-31
- ISSN
- 0009-2363 (ISSN-L)
- 1347-5223
- OpenAlex
- W2137541074
- Cited by
- 46
- References
- 17
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 2 · 2023: 1 · 2022: 6 · 2021: 3 · 2019: 2 · 2018: 2 · 2017: 4 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 5 · 2012: 2
- FWCI
- 0.88
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Chemical and Pharmaceutical Bulletin (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Physical Sciences › Chemistry › Organic Chemistry › Surfactants and Colloidal Systems
- Keywords
- PEG-liposomes, liposome permeability, DSPE-PEG, PEG molecular weight, DPPC, carboxyfluorescein leakage, DPH fluorescence polarization, TMA-DPH, gel and liquid-crystalline phases, phospholipid bilayers
- MeSH terms
- Dose-Response Relationship, Drug, Liposomes, Permeability, Polyethylene Glycols
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