The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Methods for microbial DNA extraction from soil for PCR amplification
Open accessAbstract
Amplification of DNA from soil is often inhibited by co-purified contaminants. A rapid, inexpensive, large-scale DNA extraction method involving minimal purification has been developed that is applicable to various soil types (1). DNA is also suitable for PCR amplification using various DNA targets. DNA was extracted from 100g of soil using direct lysis with glass beads and SDS followed by potassium acetate precipitation, polyethylene glycol precipitation, phenol extraction and isopropanol precipitation. This method was compared to other DNA extraction methods with regard to DNA purity and size.
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Cites
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Deininger, P. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,682
DNA recovery from soils of diverse composition
Zhou, J., Bruns, M. A., & Tiedje, J. M. (1996) · Applied and Environmental Microbiology · doi:10.1128/aem.62.2.316-322.1996 · Cited by 2,880
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The extraction and purification of microbial DNA from sediments
Ogram, A., Sayler, G. S., & Barkay, T. (1987) · Journal of Microbiological Methods · doi:10.1016/0167-7012(87)90025-x · Cited by 783
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Rapid method for direct extraction of DNA from soil and sediments
Tsai, Y. L., & Olson, B. H. (1991) · Applied and Environmental Microbiology · doi:10.1128/aem.57.4.1070-1074.1991 · Cited by 611
DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community
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Cited by
A Comprehensive Evaluation of PCR Primers to Amplify the nifH Gene of Nitrogenase
Gaby, J. C., & Buckley, D. H. (2012) · PLoS ONE · doi:10.1371/journal.pone.0042149 · Cited by 393
Kermekchiev, M. B., Kirilova, L. I., Vail, E. E., & Barnes, W. M. (2009) · Nucleic Acids Research · doi:10.1093/nar/gkn1055 · Cited by 259
Chang, H.-W., Kim, K.-H., Nam, Y.-D., Roh, S. W., Kim, M.-S., Jeon, C. O., Oh, H.-M., & Bae, J.-W. (2008) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2008.05.013 · Cited by 129
Metadata
- Authors
- Yeates, C., Gillings, M. R., Davison, A. D., Altavilla, N., & Veal, D. A.
- Venue
- Biological Procedures Online
- Published
- 1998-05
- Volume
- 1
- Issue
- 1
- Pages
- 40-47
- ISSN
- 1480-9222 (ISSN-L)
- DOI
- 10.1251/bpo6
- OpenAlex
- W2073497355
- Cited by
- 311
- References
- 24
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Yeates, C., Gillings, M. R., Davison, A. D., Altavilla, N., & Veal, D. A. (1998). Methods for microbial DNA extraction from soil for PCR amplification. Biological Procedures Online. https://doi.org/10.1251/bpo6
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
