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Rapid method for direct extraction of DNA from soil and sediments
Open accessAbstract
A rapid method for the direct extraction of DNA from soil and sediments was developed. The indigenous microorganisms in the soil and sediments were lysed by using lysozyme and a freeze-thaw procedure. The lysate was extracted with sodium dodecyl sulfate and phenol-chloroform. In addition to a high recovery efficiency (greater than 90%), the yields of DNA were high (38 and 12 micrograms/g [wet weight] from sediments and soil, respectively). This method generated minimal shearing of the extracted DNA. The crude DNA could be further purified with an Elutip-d column if necessary. An additional advantage of this method is that only 1 g of sample is required, which allows for the analysis of small samples and the processing of many samples in a relatively short (7 h) period.
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Cited by
Chee-Sanford, J. C., Aminov, R. I., Krapac, I. J., Garrigues-Jeanjean, N., & Mackie, R. I. (2001) · Applied and Environmental Microbiology · doi:10.1128/aem.67.4.1494-1502.2001 · Cited by 773
Marilley, L., & Aragno, M. (1999) · Applied Soil Ecology · doi:10.1016/s0929-1393(99)00028-1 · Cited by 317
Amplification of DNA from native populations of soil bacteria by using the polymerase chain reaction
Bruce, K. D., Hiorns, W. D., Hobman, J. L., Osborn, A. M., Strike, P., & Ritchie, D. A. (1992) · Applied and Environmental Microbiology · doi:10.1128/aem.58.10.3413-3416.1992 · Cited by 253
GONZaLEZ, J. M., MAYER, F., MORAN, M. A., HODSON, R. E., & WHITMAN, W. B. (1997) · International Journal of Systematic Bacteriology · doi:10.1099/00207713-47-2-369 · Cited by 233
Kocherginskaya, S. A., Aminov, R. I., & White, B. A. (2001) · Anaerobe · doi:10.1006/anae.2001.0378 · Cited by 214
Metadata
- Authors
- Tsai, Y. L., & Olson, B. H.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1991-04
- Volume
- 57
- Issue
- 4
- Pages
- 1070-1074
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1514657191
- Cited by
- 611
- References
- 0
- FWCI
- 30.68
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Tsai, Y. L., & Olson, B. H. (1991). Rapid method for direct extraction of DNA from soil and sediments. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.57.4.1070-1074.1991
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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