Dicer is dispensable for asymmetric RISC loading in mammals
Abstract
In flies, asymmetric loading of small RNA duplexes into Argonaute2-containing RNA-induced silencing complex (Ago2-RISC) requires Dicer-2/R2D2 heterodimer, which acts as a protein sensor for the thermodynamic stabilities of the ends of small RNA duplexes. However, the mechanism of small RNA asymmetry sensing in mammalian RISC assembly remains obscure. Here, we quantitatively examined RISC assembly and target silencing activity in the presence or absence of Dicer in mammals. Our data show that, unlike the well-characterized fly Ago2-RISC assembly pathway, mammalian Dicer is dispensable for asymmetric RISC loading in vivo and in vitro.
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Mammalian Dicer is dispensable for asymmetric miRNA strand selection in vitro.
“Furthermore, mammalian Dicer is dispensable for asymmetric miRNA strand selection in vitro (Betancur & Tomari, 2011; Murchison, Partridge, Tam, Cheloufi, & Hannon, 2005)”
From microRNA strand selection: Unwinding the rules (2021) · cites this paper as Betancur & Tomari, 2011
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Morphogenesis in skin is governed by discrete sets of differentially expressed microRNAs
Yi, R., O'Carroll, D., Pasolli, H. A., Zhang, Z., Dietrich, F. S., Tarakhovsky, A., & Fuchs, E. (2006) · Nature Genetics · doi:10.1038/ng1744 · Cited by 555 · see citing works
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Research works printed but not linked: 2
- Fo ¨rstemann K, Tomari Y, Du T, Vagin VV, Denli AM, Bratu DP, Klattenhoff C, Theurkauf WE, Zamore PD. 2005. Normal micro- RNA maturation and germ-line stem cell maintenance requires Loquacious, a double-stranded RNA-binding domain protein. PLoS Biol 3: e236. doi: 10.1371/journal.pbio.0030236. · DOI
- Saito K, Ishizuka A, Siomi H, Siomi MC. 2005. Processing of pre- microRNAs by the Dicer-1-Loquacious complex in Drosophila cells. PLoS Biol 3: e235. doi: 10.1371/journal.pbio.0030235. · DOI
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Regulation of microRNA biogenesis
Ha, M., & Kim, V. N. (2014) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3838 · Cited by 5,462 · see citing works
microRNA strand selection: Unwinding the rules
Medley, J. C., Panzade, G., & Zinovyeva, A. Y. (2021) · WIREs RNA · doi:10.1002/wrna.1627 · Cited by 225 · see citing works
- Authors
- Betancur, J. G., & Tomari, Y.
- Venue
- RNA
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2011-11-21
- Volume
- 18
- Issue
- 1
- Pages
- 24-30
- ISSN
- 1355-8382
- 1469-9001
- Cited by
- 68
- References
- 1
- Language
- en
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