The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Overcoming the bottleneck to widespread testing: a rapid review of nucleic acid testing approaches for COVID-19 detection
Open accessAbstract
The current COVID-19 pandemic presents a serious public health crisis, and a better understanding of the scope and spread of the virus would be aided by more widespread testing. Nucleic-acid-based tests currently offer the most sensitive and early detection of COVID-19. However, the “gold standard” test pioneered by the U.S. Centers for Disease Control and Prevention takes several hours to complete and requires extensive human labor, materials such as RNA extraction kits that could become in short supply, and relatively scarce qPCR machines. It is clear that a huge effort needs to be made to scale up current COVID-19 testing by orders of magnitude. There is thus a pressing need to evaluate alternative protocols, reagents, and approaches to allow nucleic-acid testing to continue in the face of these potential shortages. There has been a tremendous explosion in the number of papers written within the first weeks of the pandemic evaluating potential advances, comparable reagents, and alternatives to the “gold-standard” CDC RT-PCR test. Here we present a collection of these recent advances in COVID-19 nucleic acid testing, including both peer-reviewed and preprint articles. Due to the rapid developments during this crisis, we have included as many publications as possible, but many of the cited sources have not yet been peer-reviewed, so we urge researchers to further validate results in their own laboratories. We hope that this review can urgently consolidate and disseminate information to aid researchers in designing and implementing optimized COVID-19 testing protocols to increase the availability, accuracy, and speed of widespread COVID-19 testing.
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Citations
Cites
Loop-mediated isothermal amplification of DNA
Notomi, T. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.12.e63 · Cited by 8,805
Virological assessment of hospitalized patients with COVID-2019
Wölfel, R., Corman, V. M., Guggemos, W., Seilmaier, M., Zange, S., Müller, M. A., Niemeyer, D., Jones, T. C., Vollmar, P., Rothe, C., et al. (2020) · Nature · doi:10.1038/s41586-020-2196-x · Cited by 6,661
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
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Nucleic acid detection with CRISPR-Cas13a/C2c2
Gootenberg, J. S., Abudayyeh, O. O., Lee, J. W., Essletzbichler, P., Dy, A. J., Joung, J., Verdine, V., Donghia, N., Daringer, N. M., Freije, C. A., et al. (2017) · Science · doi:10.1126/science.aam9321 · Cited by 3,844
CRISPR–Cas12-based detection of SARS-CoV-2
Broughton, J. P., Deng, X., Yu, G., Fasching, C. L., Servellita, V., Singh, J., Miao, X., Streithorst, J. A., Granados, A., Sotomayor-Gonzalez, A., et al. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0513-4 · Cited by 2,717
DNA Detection Using Recombination Proteins
Piepenburg, O., Williams, C. H., Stemple, D. L., & Armes, N. A. (2006) · PLoS Biology · doi:10.1371/journal.pbio.0040204 · Cited by 2,482
Diagnosing COVID-19: The Disease and Tools for Detection
Udugama, B., Kadhiresan, P., Kozlowski, H. N., Malekjahani, A., Osborne, M., Li, V. Y. C., Chen, H., Mubareka, S., Gubbay, J. B., & Chan, W. C. W. (2020) · ACS Nano · doi:10.1021/acsnano.0c02624 · Cited by 1,754
CRISPR-Cas12a-assisted nucleic acid detection
Li, S.-Y., Cheng, Q.-X., Wang, J.-M., Li, X.-Y., Zhang, Z.-L., Gao, S., Cao, R.-B., Zhao, G.-P., & Wang, J. (2018) · Cell Discovery · doi:10.1038/s41421-018-0028-z · Cited by 1,530
Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay
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Wong, Y.-P., Othman, S., Lau, Y.-L., Radu, S., & Chee, H.-Y. (2018) · Journal of Applied Microbiology · doi:10.1111/jam.13647 · Cited by 712
Cited by
Considerations for diagnostic COVID-19 tests
Vandenberg, O., Martiny, D., Rochas, O., van Belkum, A., & Kozlakidis, Z. (2021) · Nature Reviews Microbiology · doi:10.1038/s41579-020-00461-z · Cited by 803
Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA
Patchsung, M., Jantarug, K., Pattama, A., Aphicho, K., Suraritdechachai, S., Meesawat, P., Sappakhaw, K., Leelahakorn, N., Ruenkam, T., Wongsatit, T., et al. (2020) · Nature Biomedical Engineering · doi:10.1038/s41551-020-00603-x · Cited by 640
From Diagnosis to Treatment: Recent Advances in Patient-Friendly Biosensors and Implantable Devices
Li, P., Lee, G.-H., Kim, S. Y., Kwon, S. Y., Kim, H.-R., & Park, S. (2021) · ACS Nano · doi:10.1021/acsnano.0c06688 · Cited by 359
Metadata
- Authors
- Esbin, M. N., Whitney, O. N., Chong, S., Maurer, A., Darzacq, X., & Tjian, R.
- Venue
- RNA
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2020-05-01
- Volume
- 26
- Issue
- 7
- Pages
- 771-783
- ISSN
- 1355-8382 (ISSN-L)
- 1469-9001
- OpenAlex
- W3023118558
- Cited by
- 477
- References
- 49
- FWCI
- 12.28
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Esbin, M. N., Whitney, O. N., Chong, S., Maurer, A., Darzacq, X., & Tjian, R. (2020). Overcoming the bottleneck to widespread testing: a rapid review of nucleic acid testing approaches for COVID-19 detection. RNA. https://doi.org/10.1261/rna.076232.120
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