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Cited by 13Chromosomal and Genetic VariationsRNA Interference and Gene DeliveryEpigenetics and DNA Methylation

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The two Gtsf paralogs in silkworms orthogonally activate their partner PIWI proteins for target cleavage

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Izumi, Natsuko, Shoji, Keisuke, Kiuchi, Takashi, Katsuma, Susumu, Tomari, Yukihide·2023·RNA
DOI 10.1261/rna.079380.122PMC PMC9808576

Published version of a preprint

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Abstract

The PIWI-interacting RNA (piRNA) pathway is a protection mechanism against transposons in animal germ cells. Most PIWI proteins possess piRNA-guided endonuclease activity, which is critical for silencing transposons and producing new piRNAs. Gametocyte-specific factor 1 (Gtsf1), an evolutionarily conserved zinc finger protein, promotes catalysis by PIWI proteins. Many animals have multiple Gtsf1 paralogs; however, their respective roles in the piRNA pathway are not fully understood. Here, we dissected the roles of Gtsf1 and its paralog Gtsf1-like (Gtsf1L) in the silkworm piRNA pathway. We found that Gtsf1 and Gtsf1L preferentially bind the two silkworm PIWI paralogs, Siwi and BmAgo3, respectively, and facilitate the endonuclease activity of each PIWI protein. This orthogonal activation effect was further supported by specific reduction of BmAgo3-bound Masculinizer piRNA and Siwi-bound Feminizer piRNA, the unique piRNA pair required for silkworm feminization, upon depletion of Gtsf1 and Gtsf1L, respectively. Our results indicate that the two Gtsf paralogs in silkworms activate their respective PIWI partners, thereby facilitating the amplification of piRNAs.

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Metadata

Authors
Izumi, N., Shoji, K., Kiuchi, T., Katsuma, S., & Tomari, Y.
Affiliations
  • Institute for Quantitative Biosciences, The University of Tokyo (JP)
  • The University of Tokyo (JP)
Corresponding author
Tomari, Yukihide
ORCID
Venue
RNA
Publisher
Cold Spring Harbor Laboratory
Published
2022-11-01
Volume
29
Issue
1
Pages
18-29
ISSN
  • 1355-8382 (ISSN-L)
  • 1469-9001
DOI
10.1261/rna.079380.122
PMC
PMC9808576
OpenAlex
W4307851397
Cited by
13
References
1
Citations by year
2026: 3 · 2025: 5 · 2024: 2 · 2023: 3
FWCI
1.38
Type
journal article
Language
en
Open access
Licence
cc-by-nc-nd
Locations
  • RNA (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Funders
  • NIH (S10 OD018174)
  • Grant-in-Aid for Scientific Research (18H05271, 19K06484)
  • Grant-in-Aid for Scientific Research on Innovative Areas (17H06431)
  • Grant-in-Aid for Early-Career Scientists (22K15082)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
Keywords
transposon silencing, Bombyx mori, germ cells, zinc finger proteins

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