The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
The PIWI proteins SMEDWI-2 and SMEDWI-3 are required for stem cell function and piRNA expression in planarians
Open accessAbstract
PIWI proteins are expressed in germ cells in a wide variety of metazoans, where they participate in the synthesis and function of a novel class of small RNAs called PIWI associated RNAs (piRNAs). One function of piRNAs is to preserve the integrity of the germline genome by silencing transposons, though they also participate in epigenetic and post-transcriptional gene regulation. In the planarian Schmidtea mediterranea, the PIWI proteins SMEDWI-1 and SMEDWI-2 are expressed in neoblasts and SMEDWI-2 is required for regeneration and homeostasis. Here, we identify a diverse population of ∼32-nucleotide small RNAs that strongly resemble vertebrate and insect piRNAs and map to hundreds of thousands of loci in the S. mediterranea genome. The expression of these RNAs occurs predominantly in neoblasts and is not restricted to the germline. RNAi knockdown of either SMEDWI-2 or a newly identified PIWI protein, SMEDWI-3, impairs regeneration and homeostasis and decreases the levels of both piRNAs and neoblasts. Therefore, SMEDWI-2 and SMEDWI-3 are required for piRNA expression, regeneration, and neoblast function in S. mediterranea.
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Cites
CAP3: A DNA Sequence Assembly Program
Huang, X., & Madan, A. (1999) · Genome Research · doi:10.1101/gr.9.9.868 · Cited by 5,261
A germline-specific class of small RNAs binds mammalian Piwi proteins
Girard, A., Sachidanandam, R., Hannon, G. J., & Carmell, M. A. (2006) · Nature · doi:10.1038/nature04917 · Cited by 1,762
Drosophila PIWI associates with chromatin and interacts directly with HP1a
Brower-Toland, B., Findley, S. D., Jiang, L., Liu, L., Yin, H., Dus, M., Zhou, P., Elgin, S. C. R., & Lin, H. (2007) · Genes & Development · doi:10.1101/gad.1564307 · Cited by 350
Cited by
MicroRNAs: key regulators of stem cells
Gangaraju, V. K., & Lin, H. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2621 · Cited by 734
Juliano, C., Wang, J., & Lin, H. (2011) · Annual Review of Genetics · doi:10.1146/annurev-genet-110410-132541 · Cited by 374
A conserved germline multipotency program
Juliano, C. E., Swartz, S. Z., & Wessel, G. M. (2010) · Development · doi:10.1242/dev.047969 · Cited by 268
The stem cell system in demosponges: Insights into the origin of somatic stem cells
Funayama, N. (2010) · Development, Growth & Differentiation · doi:10.1111/j.1440-169x.2009.01162.x · Cited by 95
Funayama, N. (2013) · Development Genes and Evolution · doi:10.1007/s00427-012-0417-5 · Cited by 82
Metadata
- Authors
- Palakodeti, D., Smielewska, M., Lu, Y.-C., Yeo, G. W., & Graveley, B. R.
- Venue
- RNA
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2008-05-02
- Volume
- 14
- Issue
- 6
- Pages
- 1174-1186
- ISSN
- 1355-8382 (ISSN-L)
- 1469-9001
- OpenAlex
- W2111086936
- Cited by
- 236
- References
- 3
- FWCI
- 6.82
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Palakodeti, D., Smielewska, M., Lu, Y.-C., Yeo, G. W., & Graveley, B. R. (2008). The PIWI proteins SMEDWI-2 and SMEDWI-3 are required for stem cell function and piRNA expression in planarians. RNA. https://doi.org/10.1261/rna.1085008
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