A germline-specific class of small RNAs binds mammalian Piwi proteins
Abstract
Small RNAs associate with Argonaute proteins and serve as sequence-specific guides to regulate messenger RNA stability, protein synthesis, chromatin organization and genome structure. In animals, Argonaute proteins segregate into two subfamilies. The Argonaute subfamily acts in RNA interference and in microRNA-mediated gene regulation using 21-22-nucleotide RNAs as guides. The Piwi subfamily is involved in germline-specific events such as germline stem cell maintenance and meiosis. However, neither the biochemical function of Piwi proteins nor the nature of their small RNA guides is known. Here we show that MIWI, a murine Piwi protein, binds a previously uncharacterized class of approximately 29-30-nucleotide RNAs that are highly abundant in testes. We have therefore named these Piwi-interacting RNAs (piRNAs). piRNAs show distinctive localization patterns in the genome, being predominantly grouped into 20-90-kilobase clusters, wherein long stretches of small RNAs are derived from only one strand. Similar piRNAs are also found in human and rat, with major clusters occurring in syntenic locations. Although their function must still be resolved, the abundance of piRNAs in germline cells and the male sterility of Miwi mutants suggest a role in gametogenesis.
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Ago3 regulates PIWI-interacting RNAs (piRNAs), a large class of highly conserved small noncoding RNAs that are responsible for silencing transposons.
“Ago3 regulates PIWI-interacting RNAs, or piRNAs, a large class of highly conserved small noncoding RNAs that are responsible for silencing transposons33,34.”
From Tau promotes neurodegeneration through global chromatin relaxation (2014) · cites this paper as [34]
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- Authors
- Girard, A., Sachidanandam, R., Hannon, G. J., & Carmell, M. A.
- Affiliations
- Cold Spring Harbor Laboratory (US)
- Corresponding author
- Hannon, Gregory J.
- Venue
- Nature
- Published
- 2006-06-04
- Volume
- 442
- Issue
- 7099
- Pages
- 199-202
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1977733607
- Cited by
- 1,762
- References
- 1
- Citations by year
- 2026: 55 · 2025: 97 · 2024: 74 · 2023: 88 · 2022: 85 · 2021: 110 · 2020: 100 · 2019: 80 · 2018: 86 · 2017: 80 · 2016: 69 · 2015: 95 · 2014: 84 · 2013: 90 · 2012: 87
- FWCI
- 41.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Keywords
- piRNA, PIWI proteins, MIWI, Argonaute proteins, RNA interference, RNA-induced silencing complex, germ cells, piRNA clusters, gametogenesis, male sterility, germline stem cells, meiosis
- MeSH terms
- Animals, Multigene Family, Humans, Male, Molecular Sequence Data, Organ Specificity, Protein Binding, Proteins, Spermatogenesis, Testis, Conserved Sequence, Synteny, MicroRNAs, Mice, Rats, Argonaute Proteins
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