The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Evidence for reassociation of RNA-binding proteins after cell lysis: Implications for the interpretation of immunoprecipitation analyses
Open accessAbstract
Immuno- and other affinity-purification approaches are commonly used to characterize the composition of ribonucleoprotein complexes. While associations detected by these procedures are often interpreted as reflecting in vivo interactions, it is also possible that they arise from reassociation of molecules after cell lysis. Here we used an experimental approach that allowed us to distinguish between these possibilities. Surprisingly, we show that the association of the RNA-binding protein HuR with its target mRNA, c-fos, as detected by co-immunoprecipitation, results largely from reassociation of molecules subsequent to cell lysis. The existence of such postlysis reassortments thus demonstrates that co-immunoprecipitation does not always recapitulate the in vivo state of ribonucleoprotein complexes.
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Brennan, C. M., & Steitz*, J. A. (2001) · Cellular and Molecular Life Sciences · doi:10.1007/pl00000854 · Cited by 1,095
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Polymenidou, M., Lagier-Tourenne, C., Hutt, K. R., Huelga, S. C., Moran, J., Liang, T. Y., Ling, S.-C., Sun, E., Wancewicz, E., Mazur, C., et al. (2011) · Nature Neuroscience · doi:10.1038/nn.2779 · Cited by 1,335
Metadata
- Authors
- MILI, S., & STEITZ, J. A.
- Venue
- RNA
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2004-09-23
- Volume
- 10
- Issue
- 11
- Pages
- 1692-1694
- ISSN
- 1355-8382 (ISSN-L)
- 1469-9001
- OpenAlex
- W2137969903
- Cited by
- 366
- References
- 1
- FWCI
- 3.50
- Type
- journal article
- Language
- en
- Open access
- bronze
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- MILI, S., & STEITZ, J. A. (2004). Evidence for reassociation of RNA-binding proteins after cell lysis: Implications for the interpretation of immunoprecipitation analyses. RNA. https://doi.org/10.1261/rna.7151404
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