Overexpression of GADD34 enhances production of recombinant human antithrombin III in Chinese hamster ovary cells
Abstract
To improve the production of recombinant human antithrombin III (AT-III) in Chinese hamster ovary (CHO) cells, the gene encoding growth arrest and DNA damage inducible protein 34 (GADD34), which is a transcription factor involved in the unfolded protein response (UPR), was cloned from CHO-K1 cells. Overexpression of GADD34 significantly enhanced the production of recombinant AT-III in CHO 13D-35D cells. The specific rate of AT-III production in the GADD34-overexpressing CHO 13D-35D cells reached approximately 28 pg/cell/d. After 144 h of incubation, the AT-III concentration in the culture supernatant was approximately 40% higher than that observed in the case of the parental CHO 13D-35D cells. The mRNA expression, specific activity, and fucosylation of AT-III were not affected by GADD34 overexpression. Overexpression of GADD34 is a promising method of improving the production of secreted protein pharmaceuticals in CHO cells.
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GADD34 can also be overexpressed in order to increase productivity of CHO cells.
“Similarly, GADD34 can also be overexpressed in order to increase productivity of CHO cells (Omasa et al., 2008).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Omasa et al., 2008
Cites
Ohya, T., Hayashi, T., Kiyama, E., Nishii, H., Miki, H., Kobayashi, K., Honda, K., Omasa, T., & Ohtake, H. (2008) · Biotechnology and Bioengineering · doi:10.1002/bit.21758 · Cited by 96 · see citing works
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Therapeutic glycoprotein production in mammalian cells
Lalonde, M.-E., & Durocher, Y. (2017) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2017.04.028 · Cited by 296 · see citing works
- Authors
- Omasa, T., Takami, T., Ohya, T., Kiyama, E., Hayashi, T., Nishii, H., Miki, H., Kobayashi, K., Honda, K., & Ohtake, H.
- Affiliations
- The University of Osaka (JP)
- Tanabe Pharma Corporation (Japan) (JP)
- Venue
- Journal of Bioscience and Bioengineering
- Publisher
- Elsevier BV
- Published
- 2008-12
- Volume
- 106
- Issue
- 6
- Pages
- 568-573
- ISSN
- 1347-4421 (ISSN-L)
- 1389-1723
- OpenAlex
- W2079933818
- Cited by
- 57
- References
- 1
- Citations by year
- 2025: 3 · 2023: 2 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 5 · 2018: 2 · 2017: 7 · 2016: 5 · 2015: 1 · 2014: 6 · 2013: 5 · 2012: 2
- FWCI
- 2.06
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Bioscience and Bioengineering (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Endoplasmic Reticulum Stress and Disease
- Keywords
- GADD34, antithrombin III, recombinant protein expression, CHO cells, unfolded protein response, transcription factor overexpression, CHO-K1 cells, fucosylation
- MeSH terms
- Animals, Antigens, Differentiation, Antithrombin III, Base Sequence, Cricetulus, Cricetinae, Humans, Recombinant Proteins, Blotting, Western, CHO Cells, DNA Primers, Cell Cycle Proteins, Protein Phosphatase 1
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