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Cited by 39Medicinal Plants and NeuroprotectionNeurological Disorders and TreatmentsNeurological Disease Mechanisms and Treatments

Asiaticoside Alleviates Cerebral Ischemia-Reperfusion Injury via NOD2/Mitogen-Activated Protein Kinase (MAPK)/Nuclear Factor kappa B (NF-κB) Signaling Pathway

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Zhang, Chunhui, Chen, Suyan, Zhang, Zhenxiang, Xu, Hui, Zhang, Weihong, Xu, Dongqing, Lin, Beilei, Mei, Yongxia·2020·Medical Science Monitor
DOI 10.12659/msm.920325PMC PMC7009775
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Abstract

BACKGROUND Cerebral ischemia-reperfusion injury (CIRI) remains a serious health problem. Centella asiatica formulations are used to treat central nervous system disorders. In the present study, asiaticoside, an extract of the plant Centella asiatica, was investigated in CIRI in vivo and vitro. MATERIAL AND METHODS We made a CIRI model in vivo in SD rats treated by middle cerebral artery occlusion, and a cell model of ischemia-reperfusion injury was made in PC12 cells treated by deprivation of oxygen and glucose/restoration. CIRI in vivo was assessed by scores of neurological functions, encephaledema, and cerebral infarction area. Inflammation level and oxidative stress level were detected by the appropriate kits. TUNEL assay was performed for assessment of cell apoptosis and Western blot analysis was performed to assess protein expression levels. CCK8 assay was performed for evaluation of cell survival and flow cytometer was used to detect cell apoptosis in vitro. RESULTS Nervous function injury, brain edema, cell apoptosis, infarct size, apoptosis-related protein expressions, and protein expressions of the NOD2/MAPK/NF-kappaB signaling pathway in the CIRI model were all reversed by asiaticoside in rats. The cell apoptosis, inflammation level, and oxidative stress level in the model of cerebral ischemia-reperfusion injury were reduced by asiaticoside. The effects of asiaticoside on CIRI were reversed by NOD 2 agonists. CONCLUSIONS Asiaticoside showed a protective effect against cerebral ischemia-reperfusion injury via the NOD2/MAPK/NF-kappaB signaling pathway. These findings are vital for future research on use of asiaticoside in CIRI, providing a new avenue for alleviating CIRI.

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  • Asiaticoside reverses nervous function injury, brain oedema, cell apoptosis, infarct size, apoptosis-related protein expressions, inflammation and oxidative stress, and increases cell survival in rat models of cerebral ischaemia/reperfusion injury by blocking the NOD2/MAPK/NF-κB signalling pathway.

    “Asiaticoside successfully reversed nervous function injury, brain oedema, cell apoptosis, infarct size, apoptosis‐related protein expressions, inflammation and oxidative stress, and also increased cell survival by blocking the NOD2/MAPK/NF‐κB signalling pathway. 66”

    From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [66]

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  1. [1]Fekadu G Chelkeba L Kebede A Risk factors, clinical presentations and predictors of stroke among adult patients admitted to stroke unit of Jimma university medical center, south west Ethiopia: prospective observational study BMC Neurol 2019 19 1 187 31390995 PMC6685251
  2. [2]Cabral NL Nagel V Conforto AB High five-year mortality rates of ischemic stroke subtypes: A prospective cohort study in Brazil Int J Stroke 2019 14 5 491 99 30299229
  3. [3]Khandelwal P Yavagal DR Sacco RL Acute Ischemic Stroke Intervention J Am Coll Cardiol 2016 67 22 2631 44 27256835
  4. [4]Novakovic R Toth G Purdy PD Review of current and emerging therapies in acute ischemic stroke J Neurointerv Surg 2009 1 1 13 26 21994100
  5. [5]Pereira VM Yilmaz H Pellaton A Current status of mechanical thrombectomy for acute stroke treatment J Neuroradiol 2015 42 1 12 20 25660218
  6. [6]Peker A Arsava EM Topçuoğlu MA Arat A Catch Plus thrombectomy device in acute stroke: initial evaluation J Neurointerv Surg 2017 9 12 1214 18 27974375
  7. [7]Bhaskar S Stanwell P Cordato D Reperfusion therapy in acute ischemic stroke: Dawn of a new era? BMC Neurology 2018 18 1 8 29338750 PMC5771207
  8. [8]Wallert M Ziegler M Wang X Alpha-Tocopherol preserves cardiac function by reducing oxidative stress and inflammation in ischemia/reperfusion injury Redox Biol 2019 26 101292 31419755 PMC6831864
  9. [9]Li Y Zhang Y Fu H Hes1 knockdown exacerbates ischemic stroke following tMCAO by increasing ER stress-dependent apoptosis via the PERK/eIF2alpha/ATF4/CHOP signaling pathway Neurosci Bull 2019 [Epub ahead of print] PMC6977800 31309426
  10. [10]Shi WZ Tian Y Li J GCN2 suppression attenuates cerebral ischemia in mice by reducing apoptosis and endoplasmic reticulum (ER) stress through the blockage of FoxO3a-regulated ROS production Biochem Biophys Res Commun 2019 516 1 285 92 31255283
  11. [11]Rasouli Vani J Taghi Mohammadi M Sarami Foroshani M Rezazade E Evaluation of the neuroprotective and antioxidant effects of Dorema aucheri extract on cerebral ischaemia-reperfusion injury in rats Pharm Biol 2019 57 1 255 62 30957616 PMC6461074
  12. [12]Liu Q Jin Z Xu Z Antioxidant effects of ginkgolides and bilobalide against cerebral ischemia injury by activating the Akt/Nrf2 pathway in vitro and in vivo Cell Stress Chaperones 2019 24 2 441 52 30815818 PMC6439064
  13. [13]Guo S Zhang YY Peng JJ Natural compound methyl protodioscin protects rat brain from ischemia/reperfusion injury through regulation of Mul1/SOD2 pathway Eur J Pharmacol 2019 849 50 58 30716316
  14. [14]Patel LJ Raval MA Patel SG Patel AJ Development and validation of stability indicating high-performance thin-layer chromatographic (HPTLC) method for quantification of asiaticoside from Centella asiatica L. and its marketed formulation J AOAC Int 2019 102 4 1014 20 30572980
  15. [15]Bobade V Bodhankar SL Aswar U Prophylactic effects of asiaticoside-based standardized extract of Centella asiatica (L.) Urban leaves on experimental migraine: Involvement of 5HT1A/1B receptors Chin J Nat Med 2015 13 4 274 82 25908624
  16. [16]Hossain S Hashimoto M Katakura M Medicinal value of asiaticoside for Alzheimer’s disease as assessed using single-molecule-detection fluorescence correlation spectroscopy, laser-scanning microscopy, transmission electron microscopy, and in silico docking BMC Complement Altern Med 2015 15 118 25880304 PMC4422550
  17. [18]Sadeghian N Shadman J Moradi A Calcitriol protects the Blood–Brain Barrier integrity against ischemic stroke and reduces vasogenic brain edema via antioxidant and antiapoptotic actions in rats Brain Res Bull 2019 150 281 89 31220552
  18. [19]Zhang Z Li X Li D Asiaticoside ameliorates beta-amyloid-induced learning and memory deficits in rats by inhibiting mitochondrial apoptosis and reducing inflammatory factors Exp Ther Med 2017 13 2 413 20 28352309 PMC5348711
  19. [20]Gopi M Arambakkam Janardhanam V Asiaticoside: Attenuation of rotenone induced oxidative burden in a rat model of hemiparkinsonism by maintaining the phosphoinositide-mediated synaptic integrity Pharmac Biochem Behav 2017 155 1 15 28238857
  20. [21]Zhu L Liu X Du L Jin Y Preparation of asiaticoside-loaded coaxially electrospinning nanofibers and their effect on deep partial-thickness burn injury Biomed Pharmacother 2016 83 33 40 27470547
  21. [22]Hou Q Li M Lu YH Burn wound healing properties of asiaticoside and madecassoside Exp Ther Med 2016 12 3 1269 74 27588048 PMC4997909
  22. [23]Chen S Yin ZJ Jiang C Asiaticoside attenuates memory impairment induced by transient cerebral ischemia-reperfusion in mice through anti-inflammatory mechanism Pharmacol Biochem Behav 2014 122 7 15 24631487
  23. [24]Xing Y Ji Q Li X Asiaticoside protects cochlear hair cells from high glucose-induced oxidative stress via suppressing AGEs/RAGE/NF-kappaB pathway Biomed Pharmacother 2017 86 531 36 28024288
  24. [25]Yingchun L Huihan W Rong Z Antitumor activity of asiaticoside against multiple myeloma drug-resistant cancer cells is mediated by autophagy induction, activation of effector caspases, and inhibition of cell migration, invasion, and STAT-3 signaling [athway Med Sci Monit 2019 25 1355 61 30785126 PMC6391856
  25. [26]Lin X Huang R Zhang S Beneficial effects of asiaticoside on cognitive deficits in senescence-accelerated mice Fitoterapia 2013 87 69 77 23562630
  26. [27]Cowan KJ Storey KB Mitogen-activated protein kinases: New signaling pathways functioning in cellular responses to environmental stress J Exp Biol 2003 206 Pt 7 1107 15 12604570
  27. [28]Son Y Cheong YK Kim NH Mitogen-activated protein kinases and reactive oxygen species: How can ROS activate MAPK pathways? J Signal Transduct 2011 2011 792639 PMC3100083 21637379
  28. [29]Kyriakis JM Avruch J Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation Physiol Rev 2001 81 2 807 69 11274345
  29. [30]Chi Q Wang D Hu X Hydrogen sulfide gas exposure induces necroptosis and promotes inflammation through the MAPK/NF-kappaB pathway in broiler spleen Oxid Med Cell Longev 2019 2019 8061823 PMC6701317 31467636

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Authors
Zhang, C., Chen, S., Zhang, Z., Xu, H., Zhang, W., Xu, D., Lin, B., & Mei, Y.
Affiliations
  • Zhengzhou University (CN)
  • Rehabilitation Department, Henan Province Hospital of Traditional Chinese Medicine (TCM), Zhengzhou, Henan, China (mainland)
Corresponding author
Xu, Hui
ORCID
Venue
Medical Science Monitor
Publisher
International Scientific Information, Inc.
Published
2020-01-17
Volume
26
Pages
e920325-e920325
ISSN
  • 1234-1010 (ISSN-L)
  • 1643-3750
DOI
10.12659/msm.920325
PMC
PMC7009775
OpenAlex
W2999668806
Cited by
39
References
1
Citations by year
2026: 4 · 2025: 4 · 2024: 4 · 2023: 6 · 2022: 8 · 2021: 8 · 2020: 3 · 2019: 1 · 2018: 1
FWCI
6.65
Type
journal article
Language
en
Open access
Licence
cc-by-nc-nd
Locations
  • Medical Science Monitor (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
National Natural Science Foundation of China (U1404814)
Topics
  • Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
  • Life Sciences › Neuroscience › Neurology › Neurological Disorders and Treatments
  • Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
Keywords
asiaticoside, Centella asiatica, cerebral ischemia-reperfusion injury, NOD2, MAPK signaling, NF-κB signaling, neuroprotection, apoptosis, neuroinflammation, oxidative stress, cerebral edema, middle cerebral artery occlusion, oxygen-glucose deprivation/reoxygenation, PC12 cells
MeSH terms
Animals, Brain Edema, Cell Survival, Inflammation, Phosphorylation, Triterpenes, Signal Transduction, Reperfusion Injury, NF-kappa B, PC12 Cells, Rats, Sprague-Dawley, Apoptosis, Oxidative Stress, Brain Infarction, Mitogen-Activated Protein Kinases, Rats, Nod2 Signaling Adaptor Protein

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