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A sampling strategy for genome sequencing the British terrestrial arthropod fauna
Open accessAbstract
The Darwin Tree of Life (DToL) project aims to sequence and assemble high-quality genomes from all eukaryote species in Britain and Ireland, with the first phase of the project concentrating on family-level coverage plus species of particular ecological, biomedical or evolutionary interest. We summarise the processes involved in (1) assessing the UK arthropod fauna and the status of individual species on UK lists; (2) prioritising and collecting species for initial genome sequencing; (3) handling methods to ensure that high-quality genomic DNA is preserved; and (4) compiling standard operating procedures for processing specimens for genome sequencing, identification verification and voucher specimen curation. We briefly explore some lessons learned from the pilot phase of DToL and the impact of the Covid-19 pandemic.
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Cites
Earth BioGenome Project: Sequencing life for the future of life
Lewin, H. A., Robinson, G. E., Kress, W. J., Baker, W. J., Coddington, J., Crandall, K. A., Durbin, R., Edwards, S. V., Forest, F., Gilbert, M. T. P., et al. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1720115115 · Cited by 962
Cited by
A DNA barcoding framework for taxonomic verification in the Darwin Tree of Life Project
Twyford, A. D., Beasley, J., Barnes, I., Allen, H., Azzopardi, F., Bell, D., Blaxter, M. L., Broad, G., Campos-Dominguez, L., Choonea, D., et al. (2024) · Wellcome Open Research · doi:10.12688/wellcomeopenres.21143.1 · Cited by 801
Self-citationGC Content Across Insect Genomes: Phylogenetic Patterns, Causes and Consequences
Kyriacou, R. G., Mulhair, P. O., & Holland, P. W. H. (2024) · Journal of Molecular Evolution · doi:10.1007/s00239-024-10160-5 · Cited by 22
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Metadata
- Authors
- Crowley, L., Allen, H., Barnes, I., Boyes, D., Broad, G. R., Fletcher, C., Holland, P. W. H., Januszczak, I., Lawniczak, M., Lewis, O. T., Macadam, C. R., Mulhair, P. O., Pereira da Conceicoa, L., Price, B. W., Raper, C., Sivell, O., & Sivess, L.
- Venue
- Wellcome Open Research
- Publisher
- F1000 Research Ltd
- Published
- 2023-03-20
- Volume
- 8
- Pages
- 123
- ISSN
- 2398-502X (ISSN-L)
- OpenAlex
- W4327909816
- Cited by
- 597
- References
- 1
- FWCI
- 44.61
- Type
- journal article
- Language
- en
- Open access
- gold
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- Crowley, L., Allen, H., Barnes, I., Boyes, D., Broad, G. R., Fletcher, C., Holland, P. W. H., Januszczak, I., Lawniczak, M., Lewis, O. T., Macadam, C. R., Mulhair, P. O., Pereira da Conceicoa, L., Price, B. W., Raper, C., Sivell, O., & Sivess, L. (2023). A sampling strategy for genome sequencing the British terrestrial arthropod fauna. Wellcome Open Research. https://doi.org/10.12688/wellcomeopenres.18925.1
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