The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
A DNA barcoding framework for taxonomic verification in the Darwin Tree of Life Project
Open accessAbstract
Biodiversity genomics research requires reliable organismal identification, which can be difficult based on morphology alone. DNA-based identification using DNA barcoding can provide confirmation of species identity and resolve taxonomic issues but is rarely used in studies generating reference genomes. Here, we describe the development and implementation of DNA barcoding for the Darwin Tree of Life Project (DToL), which aims to sequence and assemble high quality reference genomes for all eukaryotic species in Britain and Ireland. We present a standardised framework for DNA barcode sequencing and data interpretation that is then adapted for diverse organismal groups. DNA barcoding data from over 12,000 DToL specimens has identified up to 20% of samples requiring additional verification, with 2% of seed plants and 3.5% of animal specimens subsequently having their names changed. We also make recommendations for future developments using new sequencing approaches and streamlined bioinformatic approaches.
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The SILVA ribosomal RNA gene database project: improved data processing and web-based tools
Quast, C., Pruesse, E., Yilmaz, P., Gerken, J., Schweer, T., Yarza, P., Peplies, J., & Glöckner, F. O. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks1219 · Cited by 33,055
Biological identifications through DNA barcodes
Hebert, P. D. N., Cywinska, A., Ball, S. L., & deWaard, J. R. (2003) · Proceedings of the Royal Society of London. Series B: Biological Sciences · doi:10.1098/rspb.2002.2218 · Cited by 13,376
bold : The Barcode of Life Data System (http://www.barcodinglife.org)
RATNASINGHAM, S., & HEBERT, P. D. N. (2007) · Molecular Ecology Notes · doi:10.1111/j.1471-8286.2007.01678.x · Cited by 6,376
Nilsson, R. H., Larsson, K.-H., Taylor, A. F. S., Bengtsson-Palme, J., Jeppesen, T. S., Schigel, D., Kennedy, P., Picard, K., Glöckner, F. O., Tedersoo, L., et al. (2019) · Nucleic Acids Research · doi:10.1093/nar/gky1022 · Cited by 3,530
Cryptic species as a window on diversity and conservation
Bickford, D., Lohman, D. J., Sodhi, N. S., Ng, P. K. L., Meier, R., Winker, K., Ingram, K. K., & Das, I. (2007) · Trends in Ecology & Evolution · doi:10.1016/j.tree.2006.11.004 · Cited by 3,512
Guillou, L., Bachar, D., Audic, S., Bass, D., Berney, C., Bittner, L., Boutte, C., Burgaud, G., de Vargas, C., Decelle, J., et al. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks1160 · Cited by 2,362
A DNA-Based Registry for All Animal Species: The Barcode Index Number (BIN) System
Ratnasingham, S., & Hebert, P. D. N. (2013) · PLoS ONE · doi:10.1371/journal.pone.0066213 · Cited by 2,301
The integrative future of taxonomy
Padial, J. M., Miralles, A., De la Riva, I., & Vences, M. (2010) · Frontiers in Zoology · doi:10.1186/1742-9994-7-16 · Cited by 1,915
Choosing and Using a Plant DNA Barcode
Hollingsworth, P. M., Graham, S. W., & Little, D. P. (2011) · PLoS ONE · doi:10.1371/journal.pone.0019254 · Cited by 1,305
Self-citationA sampling strategy for genome sequencing the British terrestrial arthropod fauna
Crowley, L., Allen, H., Barnes, I., Boyes, D., Broad, G. R., Fletcher, C., Holland, P. W. H., Januszczak, I., Lawniczak, M., Lewis, O. T., et al. (2023) · Wellcome Open Research · doi:10.12688/wellcomeopenres.18925.1 · Cited by 597
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Metadata
- Authors
- Twyford, A. D., Beasley, J., Barnes, I., Allen, H., Azzopardi, F., Bell, D., Blaxter, M. L., Broad, G., Campos-Dominguez, L., Choonea, D., Crowley, L., Cuber, P., Cunliffe, M., Dombrowski, A., Douglas, B., Forrest, L. L., Gaya, E., Greeves, C., Griffin, C., Harley, J., Hart, M. L., Holland, P. W. H., Hollingsworth, P. M., Januszczak, I., Jones, A., Kersey, P., Kilias, E., Lawniczak, M. K. N., Lewis, O. T., Mian, S., Minotto, A., Misra, R., Mulhair, P. O., Pereira da Conceicoa, L., Price, B. W., Salatino, S., Shaw, F., Sivell, O., Sivess, L., Uhl, R., Woof, K., & Darwin Tree of Life Consortium
- Venue
- Wellcome Open Research
- Publisher
- F1000 Research Ltd
- Published
- 2024-06-24
- Volume
- 9
- Pages
- 339
- ISSN
- 2398-502X (ISSN-L)
- OpenAlex
- W4399939545
- Cited by
- 801
- References
- 26
- FWCI
- 215.28
- Type
- journal article
- Language
- en
- Open access
- gold
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- Twyford, A. D., Beasley, J., Barnes, I., Allen, H., Azzopardi, F., Bell, D., Blaxter, M. L., Broad, G., Campos-Dominguez, L., Choonea, D., Crowley, L., Cuber, P., Cunliffe, M., Dombrowski, A., Douglas, B., Forrest, L. L., Gaya, E., Greeves, C., Griffin, C., . . . Darwin Tree of Life Consortium. (2024). A DNA barcoding framework for taxonomic verification in the Darwin Tree of Life Project. Wellcome Open Research. https://doi.org/10.12688/wellcomeopenres.21143.1
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