The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
HPLC Method for Evaluation of the Free Radical-scavenging Activity of Foods by Using 1,1-Diphenyl-2-picrylhydrazyl
Abstract
An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, alpha-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rosé wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.
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Citations
Cites
Diet and Health: What Should We Eat?
Willett, W. C. (1994) · Science · doi:10.1126/science.8160011 · Cited by 1,008
Cited by
Analytical Methods Used in Determining Antioxidant Activity: A Review
Munteanu, I. G., & Apetrei, C. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22073380 · Cited by 2,119
Free radical scavenging activity of an aqueous extract of potato peel
Singh, N., & Rajini, P. S. (2004) · Food Chemistry · doi:10.1016/j.foodchem.2003.07.003 · Cited by 632
Babbar, N., Oberoi, H. S., Uppal, D. S., & Patil, R. T. (2011) · Food Research International · doi:10.1016/j.foodres.2010.10.001 · Cited by 421
Kanatt, S. R., Chander, R., & Sharma, A. (2010) · International Journal of Food Science & Technology · doi:10.1111/j.1365-2621.2009.02124.x · Cited by 357
Antioxidant activities of phlorotannins isolated from Japanese Laminariaceae
Shibata, T., Ishimaru, K., Kawaguchi, S., Yoshikawa, H., & Hama, Y. (2008) · Journal of Applied Phycology · doi:10.1007/s10811-007-9254-8 · Cited by 289
Metadata
- Authors
- YAMAGUCHI, T., TAKAMURA, H., MATOBA, T., & TERAO, J.
- Venue
- Bioscience, Biotechnology, and Biochemistry
- Publisher
- Informa UK Limited
- Published
- 1998-01
- Volume
- 62
- Issue
- 6
- Pages
- 1201-1204
- ISSN
- 0916-8451 (ISSN-L)
- 1347-6947
- OpenAlex
- W2167549919
- Cited by
- 1,107
- References
- 1
- FWCI
- 5.10
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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